@article{mbs:/content/journal/ijsem/10.1099/ijs.0.64489-0, author = "Liu, Xing-Yu and Wang, Bao-Jun and Jiang, Cheng-Ying and Liu, Shuang-Jiang", title = "Micrococcus flavus sp. nov., isolated from activated sludge in a bioreactor", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2007", volume = "57", number = "1", pages = "66-69", doi = "https://doi.org/10.1099/ijs.0.64489-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64489-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Bacterial strain LW4T was isolated from activated sludge of a wastewater-treatment bioreactor. Cells of strain LW4T were Gram-positive cocci, with a diameter of 0.7–1.0 μm. Colonies produced on LB agar plates were yellow, smooth, circular and 0.5–1.5 mm in diameter. Strain LW4T was aerobic and grew over the temperature range 26–34 °C and pH range 5–9, with optimal growth at 30.5–31.5 °C and pH 6.0–6.2. The cell-wall peptidoglycan of strain LW4T contained amino acid residues of lysine, glutamic acid, alanine, glycine and aspartic acid. The most abundant cellular fatty acids of strain LW4T were anteiso-C15 : 0 (32.15 %) and iso-C15 : 0 (31.65 %). Major respiratory quinones were MK-8(H2) (57.3 %) and MK-7(H2) (32.9 %). The DNA G+C content was 71.4 mol% (T m). 16S rRNA gene sequence analysis indicated that strain LW4T was phylogenetically related to members of the genus Micrococcus, with similarities ranging from 96.5 to 97.3 %. Levels of DNA–DNA relatedness of strain LW4T to Micrococcus luteus DSM 20030T, Micrococcus lylae DSM 20315T and Micrococcus antarcticus AS 1.2372T were 55, 48 and 36 %, respectively. Based on these results, it is concluded that strain LW4T represents a novel species of the genus Micrococcus, for which the name Micrococcus flavus sp. nov. is proposed. The type strain is strain LW4T (=CGMCC 1.5361T=JCM 14000T).", }