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f gen. nov., sp. nov., isolated from a solar saltern and sp. nov., cultured from the Sargasso Sea, belonging to the clade

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  • Authors: Dong H. Choi1,5,5 ,  Jang-Cheon Cho2,5 ,  Brian D. Lanoil3 ,  Stephen J. Giovannoni4 , Byung C. Cho1
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    • 1 1School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, Seoul 151-742, Republic of Korea 2 2Division of Life and Marine Sciences, Inha University, Incheon 402-751, Republic of Korea 3 3Department of Environmental Sciences, University of California, Riverside, CA 92521, USA 4 4Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA
  • Byung C. Cho
 [email protected]
  • First Published Online: 01 February 2007, International Journal of Systematic and Evolutionary Microbiology 57: 270-275, doi: 10.1099/ijs.0.64552-0
  • Subject: New Taxa - Proteobacteria
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  • Two strictly aerobic, Gram-negative bacteria, designated strains CL-SP27 and B5-6, were isolated from the hypersaline water of a solar saltern in Korea and from the surface water of the Sargasso Sea, respectively. The two strains were rod-shaped, non-motile and grew on marine agar 2216 as beige colonies. Phylogenetic analyses of 16S rRNA gene sequences revealed a clear affiliation of the novel strains to the family . However, the novel strains were only distantly related to members of the clade, forming a distinct lineage. Although the 16S rRNA gene sequence similarity between strains CL-SP27 and B5-6 was very high (99.6 %), DNA–DNA relatedness between the strains was 48.4 %, suggesting that the strains be categorized as two genospecies. Additionally, the two novel strains could be differentiated by DNA G+C contents, fatty acid profiles, carbon source utilization patterns, antibiotic susceptibilities and biochemical characteristics. Based on taxonomic data obtained in this study, strains CL-SP27 and B5-6 represent separate species within a novel genus of the family , for which the names gen. nov., sp. nov. (type species) and sp. nov. are proposed. The type strains of and are CL-SP27 (=KCCM 42113=JCM 13037) and B5-6 (=KCCM 42336=JCM 14009), respectively.

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strain CL-SP27 and B5-6 are AY906863 and DQ514326, respectively.

  • A table giving the major characteristics that differentiate strains CL-SP27 and B5-6 from other related members of the family is available as supplementary material in IJSEM Online.

  • †These authors contributed equally to this work.

  • ‡ Marine Environmental Research Department, Korea Ocean Research and Development Institute (KORDI), Ansan 426-744, Republic of Korea.

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