Clostridium nitrophenolicum sp. nov., a novel anaerobic p-nitrophenol-degrading bacterium, isolated from a subsurface soil sample
Suresh, K. and Prakash, D. and Rastogi, N. and Jain, R. K.,, 57, 1886-1890 (2007),
doi = https://doi.org/10.1099/ijs.0.64604-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= An obligate anaerobic, mesophilic, motile and endospore-forming bacterium, designated 1DT, was isolated from a subsurface soil sample. The young culture of strain 1DT was Gram-positive and formed oval spores that were central in position. Based on the biochemical, chemotaxonomic and physiological data, strain 1DT appears to be a member of the genus Clostridium. Strain 1DT was found to be capable of degrading p-nitrophenol (pNP) at a concentration of 0.5 mM under anaerobic conditions as revealed by HPLC analysis. The major fatty acids were C16 : 0 (28.02 %), iso-C17 : 1 I/anteiso B (23.05 %) and C14 : 0 (10.02 %). The major polar lipid content was diphosphatidylglycerol. Strain 1DT showed highest 16S rRNA gene sequence similarity to Clostridium aciditolerans JW/YJL-B3T (98.2 %) and similarity was less for Clostridium scatologenes ATCC 25775T (95.1 %), Clostridium drakei SL1T (95.0 %) and Clostridium carboxidivorans P7T (95.0 %). Phylogenetic analysis showed that it formed a coherent cluster with the species belonging to cluster I of the genus Clostridium. The DNA G+C content was 35.5 mol%. DNA–DNA hybridization analysis indicated a mean value of 36.4 % between strain 1DT and its closest relative C. aciditolerans. Several phenotypic differences from the closely related species were also revealed. On the basis of the polyphasic characteristics, strain 1DT represents a novel species of the genus Clostridium, for which the name Clostridium nitrophenolicum sp. nov. is proposed. The type strain is 1DT (=MTCC 7832T=JCM 14030T).,
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