RT Journal Article SR Electronic(1) A1 Park, Minjeong A1 Ryu, Seung Hyun A1 Vu, Thu-Huong Thi A1 Ro, Hyeon-Su A1 Yun, Pil-Yong A1 Jeon, Che OkYR 2007 T1 Flavobacterium defluvii sp. nov., isolated from activated sludge JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 2 SP 233 OP 237 DO https://doi.org/10.1099/ijs.0.64669-0 PB Microbiology Society, SN 1466-5034, AB A Gram-negative bacterium, designated strain EMB117T, was isolated from a municipal wastewater treatment plant and characterized by polyphasic taxonomy. The cells were non-spore-forming rods that showed gliding motility. Optimal growth occurred at 25–30 °C and pH 7.0–8.0. Strain EMB117T contained phosphatidylethanolamine as the predominant polar lipid, and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH). The G+C content of the genomic DNA was 33.5 mol% and the major isoprenoid quinone was MK-6. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain EMB117T belonged to the genus Flavobacterium and was most closely related to Flavobacterium johnsoniae DSM 425T (97.8 % sequence similarity). The DNA–DNA relatedness between strain EMB117T and F. johnsoniae ATCC 17061T was about 18 %. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB117T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium defluvii sp. nov. is proposed. The type strain is EMB117T (=KCTC 12612T=DSM 17963T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64669-0