1887

Abstract

Two novel strains belonging to the phylum [formerly the (CFB) group], designated Gsoil 040 and Gsoil 052, were isolated from the soil of a ginseng field in Pocheon province, South Korea. A polyphasic approach was used to characterize the taxonomic position of the novel strains. Both strains were Gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolates belong to the genus but are clearly separated from the recognized species of this genus; gene sequence similarities between the novel isolates and type strains of recognized species ranged from 91.2 to 96.5 %. One exception was found; strain Gsoil 052 and the type strain of had a gene sequence similarity of 99.6 % but had a DNA–DNA relatedness value of 38 %. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; major fatty acids, iso-C and C 5; major hydroxy fatty acid, iso-C 3-OH and major polyamine, homospermidine) supported the affiliation of both strains Gsoil 040 and Gsoil 052 to the genus . The results of physiological and biochemical tests enabled the genotypic and phenotypic differentiation of the novel strains from the other recognized species of the genus . Therefore, it is suggested that the new isolates represent two novel species, for which the names sp. nov. [type strain Gsoil 040 (=KCTC 12654=DSM 18108)] and sp. nov. [type strain Gsoil 052 (=KCTC 12592=DSM 18017)] are proposed.

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2007-07-01
2024-03-29
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