1887

Abstract

A polyphasic taxonomic study was performed on an uncharacterized Gram-positive, catalase-negative, elongated coccus-shaped bacterium isolated from a swine-manure storage pit. The bacterium, designated strain PPC9, was facultatively anaerobic and had a DNA G+C content of 44.5 mol%. Comparative 16S rRNA gene sequencing indicated that the bacterium represented a novel subline within the genus , close to but distinct from . Strain PPC9 was readily distinguished from the five recognized species of the genus by using biochemical tests and molecular genetic analysis. Based on phylogenetic and phenotypic evidence strain PPC9 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PPC9 (=CCUG 51432=NRRL B-41357).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64730-0
2007-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/4/751.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64730-0&mimeType=html&fmt=ahah

References

  1. Bailey W. R., Scott E.G. 1974 Diagnostic Microbiology , 4th edn. St Louis, MO: C. V. Mosby;
    [Google Scholar]
  2. Bryant M. P. 1972; Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr 25:1324–1328
    [Google Scholar]
  3. Collins M. D., Ash C., Farrow J. A. E., Wallbanks S., Williams A. M. 1989; 16S Ribosomal ribonucleic acid sequence analysis of lactococci and related taxa. Description of Vagococcus fluvialis gen. nov. sp. nov.. J Appl Bacteriol 67:453–460 [CrossRef]
    [Google Scholar]
  4. Hashimoto H., Noborisaka R., Yanagawa R. 1974; Distribution of motile streptococci in faeces of man and animals and in river and sea water. Jpn J Bacteriol 29:387–393 [CrossRef]
    [Google Scholar]
  5. Hespell R. B., Wolf R., Bothast R. J. 1987; Fermentation of xylans by Butyrivibrio fibrisolvens and other ruminal bacteria. Appl Environ Microbiol 53:2849–2853
    [Google Scholar]
  6. Hoyles L., Lawson P. A., Foster G., Falsen E., Ohlen M., Grainger J. M., Collins M. D. 2000; Vagococcus fessus sp. nov., isolated from a seal and a harbour porpoise. Int J Syst Evol Microbiol 50:1151–1154 [CrossRef]
    [Google Scholar]
  7. Johnson J. L. 1994; Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology . pp  656–682 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  8. Lawson P. A., Foster G., Falsen E., Ohlen M., Collins M. D. 1999; Vagococcus lutrae sp. nov., isolated from the common otter ( Lutra lutra . Int J Syst Bacteriol 49:1251–1254 [CrossRef]
    [Google Scholar]
  9. Nicholas K. B., Nicholas H. B. Jr, Deerfield D. W. II 1997; GeneDoc: analysis and visualization of genetic variation. EMBnet News 4:2 1–4 http://www.embnet.org/download/embnetnews/embnet_news_4_2.pdf
    [Google Scholar]
  10. Page R. D. M. 1996; treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  11. Pearson W. R., Lipman D. J. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441 [CrossRef]
    [Google Scholar]
  12. Pot B., Devriese L. A., Hommez J., Miry C., Vandemeulebroecke K., Kersters K., Haesebrouck F. 1994; Characterization and identification of Vagococcus fluvialis strains isolated from domestic animals. J Appl Bacteriol 77:362–369 [CrossRef]
    [Google Scholar]
  13. Rasmussen S. W. 2002; SEQtools, a software package for analysis of nucleotide and protein sequences. http://www.seqtools.dk
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  15. Schmidtke L. M., Carson J. 1994; Characteristics of Vagococcus salmoninarum isolated from diseased salmonid fish. J Appl Bacteriol 77:229–236 [CrossRef]
    [Google Scholar]
  16. Shewmaker P. L., Steigerwalt A. G., Morey R. E., Carvalho Mda G., Elliott J. A., Joyce K., Barrett T. J., Teixeira L. M., Facklam R. R. 2004; Vagococcus carniphilu s sp. nov., isolated from ground beef. Int J Syst Evol Microbiol 54, 1505–1510 [CrossRef]
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rDNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  18. Teixeira L. M., Carvalho M. G. S., Merquior V. L. C., Steigerwalt A. G., Brenner D. J., Facklam R. R. 1997; Phenotypic and genotypic characterization of Vagococcus fluvialis , including strains isolated from human sources. J Clin Microbiol 35:2778–2781
    [Google Scholar]
  19. Wallbanks S., Martinez-Murcia A. J., Fryer J. L., Philips B. A., Collins M. D. 1990; 16S rRNA sequence determination for members of the genus Carnobacterium and related lactic acid bacteria and description of Vagococcus salmoninarum sp. nov. Int J Syst Bacteriol 40:224–230 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64730-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64730-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error