1887

Abstract

Seven bacterial isolates from farmed Atlantic cod displaying chronic granulomatous disease were characterized by phenotypic and molecular taxonomic methods. The isolates were Gram-negative, facultatively intracellular, non-motile, strictly aerobic coccobacilli which produced HS from cysteine-supplemented media and are therefore phenotypically consistent with members of the genus . Comparison of 16S rRNA gene sequences and six partial housekeeping gene sequences (, , , , and ) confirmed the organism as a member of the genus , with as its closest relative (99.3 % 16S rRNA gene sequence similarity, 92.2–99.0 % housekeeping gene sequence similarity). Despite the close relationship with , isolates from Atlantic cod could be readily distinguished phenotypically and genetically from ATCC 25015. DNA–DNA hybridization studies revealed a mean reassociation value of 68 %. Thus, on the basis of phenotypic and molecular genetic evidence, we propose that the strains isolated from Atlantic cod should be recognized as subsp. subsp. nov. with the type strain 2005/50/F292-6C (=NCIMB 14265=LMG 23800). ATCC 25015 (=DSM 735) is reclassified as subsp. subsp. nov.

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2007-09-01
2024-04-19
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References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Barns S. M., Grow C. C., Okinaka R. T., Keim P., Kuske C. R. 2005; Detection of diverse new Francisella -like bacteria in environmental samples. Appl Environ Microbiol 71:5494–5500 [CrossRef]
    [Google Scholar]
  3. Clarridge J. E. III, Raich T. J., Sjosted A., Sandstrom G., Darouiche R. O., Shawar R. M., Georghiou P. R., Osting C., Vo L. 1996; Characterization of two unusual clinically significant Francisella strains. J Clin Microbiol 34:1995–2000
    [Google Scholar]
  4. Gadagkar S. R., Rosenberg M. S., Kumar S. 2005; Inferring species phylogenies from multiple genes: concatenated sequence tree versus consensus gene tree. J Exp Zoolog B Mol Dev Evol 304:64–74
    [Google Scholar]
  5. Gots J. S. 1945; The detection of penicillinase-producing properties of micro-organisms. Science 102:309 [CrossRef]
    [Google Scholar]
  6. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  7. Hollis D. G., Weaver R. E., Steigerwalt A. G., Wenger J. D., Moss C. W., Brenner D. J. 1989; Francisella philomiragia comb nov. (formerly Yersinia philomiragia ) and Francisella tularensis biogroup novicida (formerly Francisella novicida ) associated with human disease. J Clin Microbiol 27:1601–1608
    [Google Scholar]
  8. Hsieh C. Y., Tung M. C., Tu C., Chang C. D., Tsai S. S. 2006; Enzootics of visceral granulomas associated with Francisella -like organism infection in tilapia ( Oreochromis spp.). Aquaculture 254:129–138 [CrossRef]
    [Google Scholar]
  9. Jolley K. A., Feil E. J., Chan M. S., Maiden M. C. 2001; Sequence type analysis and recombinational tests (start). Bioinformatics 17:1230–1231 [CrossRef]
    [Google Scholar]
  10. Kay W., Petersen B. O., Duus J. O., Perry M. B., Vinogradov E. 2006; Characterization of the lipopolysaccharide and beta-glucan of the fish pathogen Francisella victoria . FEBS J 273:3002–3013 [CrossRef]
    [Google Scholar]
  11. Ko K. S., Lee H. K., Park M. Y., Lee K. H., Yun Y. J., Woo S. Y., Miyamoto H., Kook Y. H. 2002; Application of RNA polymerase beta-subunit gene ( rpoB ) sequences for the molecular differentiation of Legionella species. J Clin Microbiol 40:2653–2658 [CrossRef]
    [Google Scholar]
  12. Konstantinidis K. T., Ramette A., Tiedje J. M. 2006; The bacterial species definition in the genomic era. Philos Trans R Soc Lond B Biol Sci 361:1929–1940 [CrossRef]
    [Google Scholar]
  13. Larson C. L., Wicht W., Jellison W. L. 1955; An organism resembling P. tularensis from water. Public Health Rep 70:253–258 [CrossRef]
    [Google Scholar]
  14. Lopez-Cortes A., Schumann P., Pukall R., Stackebrandt E. 2006; Exiguobacterium mexicanum sp. nov. and Exiguobacterium artemiae sp. nov., isolated from the brine shrimp Artemia franciscana . Syst Appl Microbiol 29:183–190 [CrossRef]
    [Google Scholar]
  15. Nylund A., Ottem K. F., Watanabe K., Karlsbakk E., Krossoy B. 2006; Francisella sp. (Family Francisellaceae ) causing mortality in Norwegian cod ( Gadus morhua ) farming. Arch Microbiol 185:383–392 [CrossRef]
    [Google Scholar]
  16. Olsen A. B., Mikalsen J., Rode M., Alfjorden A., Hoel E., Straum-Lie K., Haldorsen R., Colquhoun D. J. 2006; A novel systemic granulomatous inflammatory disease in farmed Atlantic cod, Gadus morhua L., associated with a bacterium belonging to the genus Francisella . J Fish Dis 29:307–311 [CrossRef]
    [Google Scholar]
  17. Ottem K. F., Nylund A., Karlsbakk E., Friis-Moller A., Krossoy B. 2007; Characterization of Francisella sp., GM2212, the first Francisella isolate from marine fish, Atlantic cod ( Gadus morhua ). Arch Microbiol 187:343–350 [CrossRef]
    [Google Scholar]
  18. Posada D., Crandall K. A. 1998; modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818 [CrossRef]
    [Google Scholar]
  19. Sjöstedt A. B. 2005 Genus I. Francisella Dorofe'ev 1947, 176AL . In Bergey's Manual of Systematic Bacteriology. The Proteobacteria , vol. 2 pp 200–210 Baltimore: Williams & Wilkins;
  20. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A., Kämpfer P., Maiden M. C., Nesme X., Rossello-Mora R., Swings J. other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  21. Swofford D. L. 2002 paup*. Phylogenetic analysis using parsimony (*and other methods), version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  22. Viscidi R. P., Demma J. C. 2003; Genetic diversity of Neisseria gonorrhoeae housekeeping genes. J Clin Microbiol 41:197–204 [CrossRef]
    [Google Scholar]
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