1887

Abstract

A Gram-negative, non-motile, coccobacilli-shaped bacterium, DS-48, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain DS-48 grew optimally at 25 °C and pH 7.0–8.0 in the presence of 0.5 % (w/v) NaCl. It contained Q-10 as the predominant ubiquinone and C 7 and C 2-OH as the major fatty acids. The DNA G+C content was 69.3 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-48 fell within the genus , clustering with TH-G33 (at a bootstrap confidence level of 100 %). The levels of similarity between the 16S rRNA gene sequence of strain DS-48 and those of the type strains of recognized species were in the range 93.2–98.0 %. DNA–DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness of DS-48, revealed that this strain differs from recognized species. On the basis of phenotypic, phylogenetic and genetic data, therefore, strain DS-48 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is DS-48 (=KCTC 12874=JCM 14592).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65113-0
2007-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/11/2485.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65113-0&mimeType=html&fmt=ahah

References

  1. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. Gallego V., Sánchez-Porro C., García M. T., Ventosa A. 2006; Roseomonas aquatica sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 56:2291–2295 [CrossRef]
    [Google Scholar]
  4. Han X. Y., Pham A. S., Tarrand J. J., Rolston K. V., Helsel L. O., Levett P. N. 2003; Bacteriologic characterization of 36 strains of Roseomonas species and proposal of Roseomonas mucosa sp nov and Roseomonas gilardii subsp rosea subsp nov. Am J Clin Pathol 120:256–264 [CrossRef]
    [Google Scholar]
  5. Helsel L. O., Hollis D. G., Steigerwalt A. G., Levett P. N. 2006; Reclassification of Roseomonas fauriae Rihs et al. 1998 as a later heterotypic synonym of Azospirillum brasilense Tarrand et al. 1979. Int J Syst Evol Microbiol 56:2753–2755 [CrossRef]
    [Google Scholar]
  6. Jiang C.-Y., Dai X., Wang B.-J., Zhou Y.-G., Liu S.-J. 2006; Roseomonas lacus sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56:25–28 [CrossRef]
    [Google Scholar]
  7. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  8. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  9. Rihs J. D., Brenner D. J., Weaver R. E., Steigerwalt A. G., Hollis D. G., Yu V. L. 1993; Roseomonas , a new genus associated with bacteremia and other human infections. J Clin Microbiol 31:3275–3283
    [Google Scholar]
  10. Sasser M. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids . Technical Note 101: Newark, DE: MIDI;
    [Google Scholar]
  11. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  12. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  13. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  14. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  15. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  16. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65113-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65113-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error