RT Journal Article SR Electronic(1) A1 Ryu, Seung Hyun A1 Park, Minjeong A1 Jeon, Yeji A1 Lee, Jung Ro A1 Park, Woojun A1 Jeon, Che OkYR 2007 T1 Flavobacterium filum sp. nov., isolated from a wastewater treatment plant in Korea JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 9 SP 2026 OP 2030 DO https://doi.org/10.1099/ijs.0.65138-0 PB Microbiology Society, SN 1466-5034, AB A Gram-negative bacterium, designated strain EMB34T, was isolated from a wastewater treatment plant in Korea. Growth was observed between 10 and 40 °C (optimum, 25–35 °C) and between pH 6.0 and 9.5 (optimum, pH 7.5–8.0). The cells were non-motile rods, linked with extracellular fibrils. The predominant fatty acids of strain EMB34T were iso-C15 : 0, C15 : 0, iso-C15 : 1 G, iso-C16 : 0 3-OH and iso-C15 : 0 3-OH and the strain contained phosphatidylethanolamine and phosphatidylinositol as the polar lipids. The G+C content of the genomic DNA was 34.2 mol% and the major quinone was menaquinone-6. Comparative 16S rRNA gene sequence analyses showed that strain EMB34T formed a distinct phyletic line within the genus Flavobacterium. The levels of 16S rRNA gene sequence similarity with other Flavobacterium species were less than 94.5 %. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain EMB34T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium filum sp. nov. is proposed. The type strain is EMB34T (=KCTC 12610T=DSM 17961T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65138-0