1887

Abstract

Three Gram-negative, rod-shaped, non-spore-forming bacteria (strains CCUG 52769, CCUG 52770 and CCUG 52771) isolated from haemodialysis water were characterized taxonomically, together with five strains isolated from industrial waters (CCUG 52428, CCUG 52507, CCUG 52575, CCUG 52590 and CCUG 52631). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the class and were related to the genus , with 16S rRNA gene sequence similarities higher than 99 % with the only species of the genus, and to sp. DSM 2583. The type strains of and were their closest neighbours (97.9 and 97.3 % 16S rRNA gene sequence similarity, respectively). Phylogenetic analysis was also performed for the internally transcribed spacer region and for three genes [ (hydrogenase), / (Rubisco) and (nitrogenase)] relevant for the metabolism of the genus . DNA–DNA hybridization, major fatty acid composition and phenotypical analyses were carried out, which included the type strain of obtained from different culture collections (ATCC 15946, CCUG 32988, DSM 654, IAM 14368 and LMG 2256), as well as IAM 14711 and CCUG 52219. Results of DNA–DNA hybridization, physiological and biochemical tests supported the conclusion that strains CCUG 52769, CCUG 52770 and CCUG 52771 represent a homogeneous phylogenetic and genomic group, including strain DSM 2583, clearly differentiated from the industrial water isolates and from the type strain. On the basis of phenotypic and genotypic characteristics, these strains belong to two novel species within the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains of sp. nov. and sp. nov. are CCUG 52769 (=CECT 7234) and CCUG 52575 (=CECT 7233), respectively.

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2007-11-01
2024-03-28
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