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Volume 57, Issue 11

Taxonomic heterogeneity, as shown by siderotyping, of strains primarily identified as Free

Abstract

One hundred and forty-four fluorescent pseudomonad strains isolated from various environments (soil, water, plant rhizosphere, hospital) and received as (83 strains), biovar A (49 strains), biovar B (10 strains) and biovar C (2 strains), were analysed by the pyoverdine-isoelectrofocusing and pyoverdine-mediated iron uptake methods of siderotyping. Both methods demonstrated a great diversity among these strains, which could be subdivided into 35 siderovars. Some siderovars specifically included strains that have subsequently been transferred to well-defined species, e.g. or , or which could be related by their siderotype to or . Other siderovars included strains sharing a high level of DNA-DNA relatedness (>70 %), thus demonstrating that siderotyping could easily circumscribe strains at the species level. However, a group of seven strains, including the type strain, ATCC 12633, were allocated into four siderovars, despite sharing DNA–DNA relatedness values of higher than 70 %. Interestingly, the strong genomic relationships between these seven strains were supported by the structural relationships among their pyoverdines, thus reflecting their phylogenetic affinities. These results strongly support the view that pyoverdine-based siderotyping could be used as a powerful tool in taxonomy.

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Keyword(s): bv., biovar , pHi, isoelectric pH , PVD, pyoverdine , PVD-IEF, pyoverdine-isoelectrofocusing and sv., siderovar
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2007-11-01
2024-04-19
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Taxonomic heterogeneity, as shown by siderotyping, of strains primarily identified as Pseudomonas putida
Int J Syst Evol Microbiol 57, 2543 (2007); https://doi.org/10.1099/ijs.0.65233-0
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