1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 5

sp. nov., isolated from a -dominated wastewater treatment system in Korea Free

Abstract

A Gram-positive, rod-shaped, spore-forming bacterium, strain S-11, was isolated from the activated sludge of a -dominated wastewater treatment system in South Korea and was characterized using a polyphasic approach in order to determine its taxonomic position. Cells (0.5–0.6×2.0–2.2 μm) were motile by means of a single subpolar flagellum. They bore ellipsoidal endospores that lay in a central position in swollen sporangia. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S-11 was a member of the genus . 16S rRNA gene sequence similarity values and DNA–DNA relatedness of strain S-11 to the type strains of other species were less than 96.2 and 66.0 %, respectively. Strain S-11 showed distinct differences in the G+C content of the genomic DNA (33.6 mol%). The major cellular fatty acids were iso-C, iso-C, anteiso-C and iso-C. The major isoprenoid quinone was MK-7. There were also some physiological differences in comparison with the type strains of species: tests for production of acetoin and acid production from dulcitol, erythritol, -inositol and sorbitol were positive. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain S-11 from the six species and subspecies with validly published names. Strain S-11 therefore represents a novel species, for which the name sp. nov. is proposed, with the type strain S-11 (=KCTC 13061 =CCUG 53534 =CIP 109363).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65335-0
2008-05-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/5/1109.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65335-0&mimeType=html&fmt=ahah

References

  1. Choi, Y. S., Hong, S. W., Kim, S. J. & Chung, I. H.(2002). Development of a biological process for livestock wastewater treatment using a technique for predominant outgrowth of Bacillus species. Water Sci Technol 45, (12). 71–78. [Google Scholar]
  2. Collins, M. D.(1985). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366. [Google Scholar]
  3. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1989).phylip – phylogeny inference package (version 3.2). Cladistics 5, 164–166. [Google Scholar]
  6. Heyrman, J., Logan, N. A., Busse, H.-J., Balcaen, A., Lebbe, L., Rodriguez-Diaz, M., Swings, J. & De Vos, P.(2003).Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int J Syst Evol Microbiol 53, 501–511.[CrossRef] [Google Scholar]
  7. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  8. Kim, Y.-G., Choi, D. H., Hyun, S. & Cho, B. C.(2007).Oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core. Int J Syst Evol Microbiol 57, 409–413.[CrossRef] [Google Scholar]
  9. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  10. Lee, J.-S., Lim, J.-M., Lee, K. C., Lee, J.-C., Park, Y.-H. & Kim, C.-J.(2006).Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions. Int J Syst Evol Microbiol 56, 251–257.[CrossRef] [Google Scholar]
  11. Lu, J., Nogi, Y. & Takami, H.(2001).Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205, 291–297.[CrossRef] [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  13. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, K. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  14. Raats, D. & Halpern, M.(2007).Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass. Int J Syst Evol Microbiol 57, 255–259.[CrossRef] [Google Scholar]
  15. Romano, I., Lama, L., Nicolaus, B. & Poli, A.(2006).Oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchi. Int J Syst Evol Microbiol 56, 805–810.[CrossRef] [Google Scholar]
  16. Rosselló-Mora, R. & Amann, R.(2001). The species concept for prokaryotes. FEMS Microbiol Rev 25, 39–67.[CrossRef] [Google Scholar]
  17. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  18. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  19. Thompson, J. D., Gilson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  20. Yumoto, I., Hirota, K., Nodasaka, Y. & Nakajima, K.(2005).Oceanobacillus oncorhynchi sp. nov., a halotolerant obligate alkaliphile isolated from the skin of a rainbow trout (Oncorhynchus mykiss), and emended description of the genus Oceanobacillus. Int J Syst Evol Microbiol 55, 1521–1524.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65335-0
Loading
Oceanobacillus caeni sp. nov., isolated from a Bacillus-dominated wastewater treatment system in Korea
Int J Syst Evol Microbiol 58, 1109 (2008); https://doi.org/10.1099/ijs.0.65335-0
/content/journal/ijsem/10.1099/ijs.0.65335-0
/content/journal/ijsem/10.1099/ijs.0.65335-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 1109 - 1113

Fatty acid compositions (%) of strain S-11 and type strains of related species.

Maximum-parsimony (Fig. S1) and maximum-likelihood (Fig. S2) trees based on 16S rRNA gene sequences of strain S-11 , type strains of other species and subspecies and related taxa.

[PDF file of Supplementary Table S1 and Figs S1 and S2](134 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error