RT Journal Article SR Electronic(1) A1 Nunoura, Takuro A1 Oida, Hanako A1 Miyazaki, Masayuki A1 Suzuki, YoheyYR 2008 T1 Thermosulfidibacter takaii gen. nov., sp. nov., a thermophilic, hydrogen-oxidizing, sulfur-reducing chemolithoautotroph isolated from a deep-sea hydrothermal field in the Southern Okinawa Trough JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 3 SP 659 OP 665 DO https://doi.org/10.1099/ijs.0.65349-0 PB Microbiology Society, SN 1466-5034, AB A novel thermophilic, sulfur-reducing chemolithoautotroph, strain ABI70S6T, was isolated from a deep-sea hydrothermal field at the Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain ABI70S6T were motile rods, 0.9–2.0 μm in length and 0.4–0.8 μm in width. Strain ABI70S6T was an obligately anaerobic chemolithotroph, exhibiting hydrogen oxidation coupled with sulfur reduction. Growth was observed at 55–78 °C (optimum, 70 °C), pH 5.0–7.5 (optimum, pH 5.5–6.0) and 0.5–4.5 % NaCl (optimum, 3.0 % NaCl). H2 and elemental sulfur were utilized as electron donor and acceptor, respectively. The major fatty acids were C16 : 0 (40.0 %) and C20 : 1 (60.0 %). The G+C content of genomic DNA was 44.2 mol%. The physiological attributes of strain ABI70S6T are similar to those of species of genera within the family Desulfurobacteriaceae, most of which are thermophilic and chemolithoautotrophic sulfur reducers. However, 16S rRNA gene sequence similarities between the novel isolate and type strains of all species within the family Desulfurobacteriaceae were <87 %, which is close to the similarities found between the novel isolate and members of the family Thermodesulfobacteriaceae (<85 %). Based on physiological and phylogenetic features of the novel isolate, it is proposed that it represents a novel species in a novel genus, Thermosulfidibacter takaii gen. nov., sp. nov., within the phylum Aquificae. The type strain of T. takaii is ABI70S6T (=JCM 13301T=DSM 17441T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65349-0