@article{mbs:/content/journal/ijsem/10.1099/ijs.0.65635-0, author = "Song, Lei and Dong, Xiuzhu", title = "Clostridium amylolyticum sp. nov., isolated from H2-producing UASB granules", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2008", volume = "58", number = "9", pages = "2132-2135", doi = "https://doi.org/10.1099/ijs.0.65635-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65635-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "UASB, upflow anaerobic sludge blanket", abstract = "A Gram-stain-positive, strictly anaerobic, mesophilic, amylolytic, rod-shaped bacterium, designated strain SW408T, was isolated from a laboratory-scale H2-producing upflow anaerobic sludge blanket reactor. The strain grew at 24–45 °C (no growth at or below 22 °C or at or above 47 °C), with optimum growth at 37 °C. The pH range for growth was 4.0–9.0 (no growth at or below pH 3.6 or at or above pH 9.3), with optimum growth at pH 7.0. Starch, cellobiose, glucose, fructose, galactose, lactose, maltose, mannose, ribose and sucrose supported growth. The major end products from glucose fermentation were ethanol, acetate, hydrogen and carbon dioxide. Abundant H2 was produced from starch fermentation. The DNA G+C content was 33.1 mol% (T m method). Phylogenetic analysis based on 16S rRNA gene sequence analysis showed that the bacterium represents a previously unrecognized species within Clostridium rRNA cluster I and is most closely related to the type strain of Clostridium frigidicarnis (94.9 % similarity). On the basis of phenotypic, genotypic and phylogenetic characteristics, strain SW408T was identified as a representative of a novel species of the genus Clostridium, for which the name Clostridium amylolyticum sp. nov. is proposed. The type strain is SW408T (=JCM 14823T=AS 1.5069T=CGMCC 1.5069T).", }