Nosocomiicoccus ampullae gen. nov., sp. nov., isolated from the surface of bottles of saline solution used in wound cleansing
Alves, Marta and Nogueira, Célia and de Magalhães-Sant'Ana, Augusto and Chung, Ana Paula and Morais, Paula V. and da Costa, Milton S.,, 58, 2939-2944 (2008),
doi = https://doi.org/10.1099/ijs.0.65753-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Strains TRF-1T and TC-9 were isolated from transfer spikes of two separate bottles containing 0.9 % NaCl (physiological saline) solution used repeatedly to wash wounds in hospital wards, months apart. Phylogenetic analysis of 16S rRNA gene sequences revealed that strains TRF-1T and TC-9 formed a distinct branch within the family Bacillaceae most closely related to the members of the genus Jeotgalicoccus. The two strains, with identical 16S rRNA gene sequences, showed sequence similarities of 89.8–93.9 % with species of Jeotgalicoccus, 91.1–91.8 % with species of Salinicoccus and 89.1–89.7 % with species of Staphylococcus. Strains TRF-1T and TC-9 are Gram-positive, non-spore-forming and non-motile cocci, with an optimum growth temperature of about 37 °C. Strain TRF-1T grew optimally in medium containing 3 % (w/v) NaCl (maximum about 14 % NaCl), while strain TC-9 grew optimally in medium with 1 % (w/v) NaCl. Both strains produce a brown pigment when grown in the presence of NaCl. The cell-wall peptidoglycan is of the A3α type with a cross-linkage containing the peptide l-Lys–Gly4–l-Ser–Gly. The major respiratory quinones are menaquinone 7 (MK-7) and menaquinone 8 (MK-8), the major fatty acids are straight-chain C14 : 0 and C16 : 0 (more than 85 % of the total) and the major polar lipid is an unknown aminophospholipid. The DNA G+C content is 33.5 mol%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics, we are of the opinion that strains TRF-1T and TC-9 represent a novel species of a new genus, for which we propose the name Nosocomiicoccus ampullae gen. nov., sp. nov. The type strain of Nosocomiicoccus ampullae is strain TRF-1T (=LMG 24060T =CIP 109506T).,
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