1887

Abstract

A Gram-negative, strictly aerobic, non-motile, club-shaped bacterial strain, designated CL-ES2, was isolated from coastal water from the east coast of Korea. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain CL-ES2 was related to the genera (95.0–96.6 % similarity to the type strains), (96.1 %) and (95.6 %) in the family . However, strain CL-ES2 did not form a robust clade with any species of the clade, instead forming a distinct subline. The optimum temperature and pH for growth were 25 °C and pH 7. Strain CL-ES2 was able to grow with sea salts at concentrations in the range 2–6 %, with optimum growth occurring at 3–4 %. The major fatty acid was C 7 (75.2 %). The polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and three unidentified lipids. The isoprenoid quinone was Q-10. The G+C content of the DNA was 47.0 mol%. On the basis of the data from the polyphasic analysis, strain CL-ES2 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is CL-ES2 (=KCCM 42274 =DSM 18290).

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2008-09-01
2024-03-28
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Neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, for strain CL-ES2 and most members of the family . [ PDF] 71 KB

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Polar lipid composition of strain CL-ES2 . The TLC plate was sprayed with molybdatophosphoric acid. AL, Unidentified aminolipid; L1–L3, unidentified lipids; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol.

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Cells of strain CL-ES2 in exponential phase (a) and stationary phase (d) stained with DAPI. [ PDF] 56 KB

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Whole-cell fatty acid compositions of strain CL-ES2 and members of the genera , and . [ PDF] 42 KB

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