1887

Abstract

A coryneform bacterium isolated from a cow with a profound ulceration was characterized by using phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and short-chain mycolic acids consistent with the genus . Comparative 16S rRNA gene sequencing showed that the organism formed a hitherto unknown subline within the genus . Sequence divergence values of greater than 3.6 % from recognized species, together with phenotypic differences, showed that the unidentified bacterium represents a new member of this genus. On the basis of phenotypic and phylogenetic considerations, it is proposed that the novel bacterium be classified as representing a novel species of the genus , with the name sp. nov. The type strain is IMMIB L-1395 (=DSM 45146=CCUG 55727).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65832-0
2009-01-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/1/34.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65832-0&mimeType=html&fmt=ahah

References

  1. Becker, B., Lechevalier, M. P., Gordon, R. E. & Lechevalier, H. A.(1964). Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole cell hydrolysates. Appl Microbiol 12, 421–423. [Google Scholar]
  2. Collins, M. D., Hoyles, L., Foster, G., Sjödén, B. & Falsen, E.(2001).Corynebacterium capitovis sp. nov., from a sheep. Int J Syst Evol Microbiol 51, 857–860.[CrossRef] [Google Scholar]
  3. Collins, M. D., Hoyles, L., Foster, G. & Falsen, E.(2004).Corynebacterium caspium sp. nov., from a Caspian seal (Phoca caspica). Int J Syst Evol Microbiol 54, 925–928.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Fernández-Garayzábal, J. F., Collins, M. D., Hutson, R. A., González, I., Fernández, E. & Domínguez, L.(1998).Corynebacterium camporealensis sp. nov., associated with subclinical mastitis in sheep. Int J Syst Bacteriol 48, 463–468.[CrossRef] [Google Scholar]
  7. Fernández-Garayzábal, J. F., Vela, A. I., Egido, R., Hutson, R. A., Lanzarot, M. P., Fernández-García, M. & Collins, M. D.(2004).Corynebacterium ciconiae sp. nov., isolated from the trachea of black storks (Ciconia nigra). Int J Syst Evol Microbiol 54, 2191–2195.[CrossRef] [Google Scholar]
  8. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  9. Funke, G., von Graevenitz, A., Clarridge, J. E., III & Bernard, K. A.(1997). Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 10, 125–159. [Google Scholar]
  10. Funke, G., Osorio, C. R., Frei, R., Riegel, P. & Collins, M. D.(1998).Corynebacterium confusum sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 48, 1291–1296.[CrossRef] [Google Scholar]
  11. Goyache, J., Ballesteros, C., Vela, A. I., Collins, M. D., Briones, V., Hutson, R. A., Potti, J., García-Borboroglu, P., Domínguez, L. & Fernández-Garayzábal, J. F.(2003).Corynebacterium sphenisci sp. nov., isolated from wild penguins. Int J Syst Evol Microbiol 53, 1009–1012.[CrossRef] [Google Scholar]
  12. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mamalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  13. Lechevalier, M. P.(1968). Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med 71, 934–944. [Google Scholar]
  14. Lechevalier, M. P. & Lechevalier, H. A.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  15. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  16. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M.(1980). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–223.[CrossRef] [Google Scholar]
  17. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  18. Renaud, F. N. R., Aubel, D., Riegel, P., Meugnier, H. & Bollet, C.(2001).Corynebacterium freneyi sp. nov., α-glucosidase-positive strains related to Corynebacterium xerosis. Int J Syst Evol Microbiol 51, 1723–1728.[CrossRef] [Google Scholar]
  19. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  20. Sjödén, B., Funke, G., Izquierdo, A., Åkervall, E. & Collins, M. D.(1998). Description of some coryneform bacteria isolated from human clinical specimens as Corynebacterium falsenii sp. nov. Int J Syst Bacteriol 48, 69–74.[CrossRef] [Google Scholar]
  21. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  22. Yassin, A. F., Steiner, U. & Ludwig, W.(2002a).Corynebacterium aurimucosum sp. nov. and emended description of Corynebacterium minutissimum Collins and Jones (1983). Int J Syst Evol Microbiol 52, 1001–1005.[CrossRef] [Google Scholar]
  23. Yassin, A. F., Steiner, U. & Ludwig, W.(2002b).Corynebacterium appendicis sp. nov. Int J Syst Evol Microbiol 52, 1165–1169.[CrossRef] [Google Scholar]
  24. Yassin, A. F., Chen, W. M., Hupfer, H., Siering, C., Kroppenstedt, R. M., Arun, A. B., Lai, W. A., Shen, F. T., Rekha, P. D. & Young, C. C.(2007).Lysobacter defluvii sp. nov., isolated from municipal solid waste. Int J Syst Evol Microbiol 57, 1131–1136.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65832-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65832-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp. 34 - 37

Extended neighbour-joining phylogenetic tree based on 16S rRNA gene sequences. [PDF](24 KB)



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error