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Volume 65, Issue Pt_10

sp. nov., isolated from intertidal sand, and emended description of the genus Free

Abstract

A novel Gram-reaction-negative, aerobic, pale-orange-pigmented bacterium, designated strain SM1216, was isolated from Arctic intertidal sand. Cells of strain SM1216 were dimorphic rods with a single polar prostheca or flagellum. The strain grew at 4 − 30 °C (optimum at 25 °C) and with 0.5 − 6 % (w/v) NaCl (optimum with 2 − 3 %). It reduced nitrate to nitrite but did not hydrolyse gelatin, DNA or Tween 80. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM1216 was affiliated with the genus in the family , sharing 97.5 and 96.3 % similarity with 14A-2-7 and 0C-2-2, respectively, the two known species in the genus . However, the level of DNA–DNA relatedness between strain SM1216 and the type strain of , the nearest phylogenetic neighbour, was 57.9 %. The major cellular fatty acids of strain SM1216 were Cω7 and C 2-OH. The main polar lipids of strain SM1216 were monoglycosyldiglyceride (MGDG), glucuronopyranosyldiglyceride (GUDG), phosphatidylglycerol (PG) and three unidentified phospholipids (PL1–3). The major respiratory quinone was ubiquinone 10 (Q10). The genomic G+C content of strain SM1216 was 60.6 mol%. On the basis of the evidence from this polyphasic study, strain SM1216 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is SM1216 ( = MCCC 1K00233 = KCTC 32513). An emended description of the genus is also given.

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2015-10-01
2024-04-20
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References

  1. Alain K., Tindall B.J., Intertaglia L., Catala P., Lebaron P. ( 2008;). Hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the Mediterranean Sea. Int J Syst Evol Microbiol 58 2511–2519 [View Article] [PubMed].
     [Google Scholar]
  2. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. ( 1997;). Gapped blast psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25 3389–3402 [View Article] [PubMed].
     [Google Scholar]
  3. Buck J.D. ( 1982;). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44 992–993.
     [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133–142 [View Article] [PubMed].
     [Google Scholar]
  5. Dong X.-Z., Cai M.-Y.(editors). ( 2001;). Determination of biochemical properties. . In Manual for the Systematic Identification of General Bacteria, pp. 370–398 Beijing: Science Press (in Chinese);.
     [Google Scholar]
  6. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376 [View Article] [PubMed].
     [Google Scholar]
  7. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–791 [View Article].
     [Google Scholar]
  8. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416 [View Article].
     [Google Scholar]
  9. Fukui Y., Abe M., Kobayashi M., Saito H., Oikawa H., Yano Y., Satomi M. ( 2013a;). Algimonas porphyrae gen. nov., sp. nov., a member of the family Hyphomonadaceae, isolated from the red alga Porphyra yezoensis . Int J Syst Evol Microbiol 63 314–320 [View Article] [PubMed].
     [Google Scholar]
  10. Fukui Y., Kobayashi M., Saito H., Oikawa H., Yano Y., Satomi M. ( 2013b;). Algimonas ampicilliniresistens sp. nov., isolated from the red alga Porphyra yezoensis, and emended description of the genus Algimonas . Int J Syst Evol Microbiol 63 4407–4412 [PubMed]. [CrossRef]
    [Google Scholar]
  11. Huß V.A., Festl H., Schleifer K.H. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4 184–192 [View Article] [PubMed].
     [Google Scholar]
  12. Jung J.Y., Kim J.M., Jin H.M., Kim S.Y., Park W., Jeon C.O. ( 2011;). Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach. Int J Syst Evol Microbiol 61 1534–1538 [View Article] [PubMed].
     [Google Scholar]
  13. Kang H.S., Lee S.D. ( 2009;). Ponticaulis koreensis gen. nov., sp. nov., a new member of the family Hyphomonadaceae isolated from seawater. Int J Syst Evol Microbiol 59 2951–2955 [View Article] [PubMed].
     [Google Scholar]
  14. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721. [CrossRef]
    [Google Scholar]
  15. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111–120 [View Article] [PubMed].
     [Google Scholar]
  16. Komagata K., Suzuki K. ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19 161–207 [View Article].
     [Google Scholar]
  17. Lane D.J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;.
     [Google Scholar]
  18. Lee S.H., Shim J.K., Kim J.M., Choi H.-K., Jeon C.O. ( 2011;). Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . Int J Syst Evol Microbiol 61 722–727 [View Article] [PubMed].
     [Google Scholar]
  19. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3 208–218 [View Article].
     [Google Scholar]
  20. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109–118 [View Article] [PubMed].
     [Google Scholar]
  21. Murray R.G.E., Doetsch R.N., Robinow C.F. ( 1994;). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  22. Ruiz-Ponte C., Cilia V., Lambert C., Nicolas J.L. ( 1998;). Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus . Int J Syst Bacteriol 48 537–542 [View Article] [PubMed].
     [Google Scholar]
  23. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 [PubMed].
     [Google Scholar]
  24. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E, Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  25. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note: a place for DNA − DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846–849 [View Article].
     [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed].
     [Google Scholar]
  27. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463–464 [View Article].
     [Google Scholar]
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Algimonas arctica sp. nov., isolated from intertidal sand, and emended description of the genus Algimonas
Int J Syst Evol Microbiol 65, 3256 (2015); https://doi.org/10.1099/ijsem.0.000402
/content/journal/ijsem/10.1099/ijsem.0.000402
/content/journal/ijsem/10.1099/ijsem.0.000402
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