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Abstract

A Gram-stain-positive, aerobic, straight or slightly bent rod-shaped, non-motile, non-spore-forming bacterium, designated strain CC5-806, was isolated from a soil sample collected from a wild karst cave in the Wulong region, Chongqing, PR China and examined using a polyphasic approach to clarify its taxonomic position. This bacterium did not produce substrate mycelium or aerial hyphae, and no diffusible pigments were observed on the media tested. Strain CC5-806 grew optimally without NaCl at 20 °C and at pH 7.0. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain CC5-806 belonged to the family and showed the highest levels of 16S rRNA gene sequence similarities with EGI 6500707 (97.56 %), 801 (97.53 %) and MP203 (97.42 %). Phylogenetic trees revealed that strain CC5-806 did not show a clear affiliation to any genus within the family . The DNA G+C content of strain CC5-806 was 62.6 mol%. The cell-wall peptidoglycan contained -lysine as a diagnostic diamino acid. The predominant menaquinones were MK-11, MK-10 and MK-9. Phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid, four unidentified phospholipids and other polar lipids were detected in the polar lipid extracts. The major fatty acids were anteiso-C, iso-C and iso-C. On the basis of the phylogenetic analysis, and phenotypic and chemotaxonomic characteristics, strain CC5-806 was distinguishable from phylogenetically related genera in the family . It represents a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is CC5-806 ( = DSM 27960 = CGMCC 1.14983).

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2015-10-01
2024-03-28
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