1887

Abstract

A Gram-stain-positive, oxidase- and catalase-negative, rod-shaped, facultatively anaerobic bacterial strain, DCY75, was isolated from a queen wasp (). Growth occurred at 4–37 °C (optimum, 30 °C), at pH 3.5–8.0 (optimum, pH 5.0–6.0) and with ≤ 7.0 % (w/v) NaCl. Strain DCY75 produced gas during growth on glucose. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DCY75 belonged to the genus and was closely related to ATCC 27651 and DSM 20690 at sequence similarities of 96.7 and 96.4 %, respectively. A comparison of two housekeeping genes, and , revealed that strain DCT75 was well separated from other species of the genus . Strain DCY75 produced - and -lactic acid isomers in a ratio of 22.5 : 77.5 (v/v). The major fatty acids were summed feature 8 (comprising C ω7 and/or Cω6), C, Cω9 and C.The peptidoglycan structure was of the A4α (-Lys–-Asp) type. Cell-wall sugars were glucose, galactose and ribose. The DNA G+C content was 35.5 ± 1.3 mol%. Based on phenotypic and genotypic properties, strain DCY75 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DCY75 ( = KCTC 21023 = JCM 19742).

Funding
This study was supported by the:
  • Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries (Award 313038-03-1-SB010)
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2015-10-01
2024-04-20
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References

  1. Anzai Y., Kim H., Park J.Y., Wakabayashi H., Oyaizu H. ( 2000;). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50 15631589 [View Article] [PubMed].
    [Google Scholar]
  2. Back W., Bohak I., Ehrmann M., Ludwig W., Schleifer K.H. ( 1996;). Revival of the species Lactobacillus lindneri and the design of a species specific oligonucleotide probe. Syst Appl Microbiol 19 322325 [View Article].
    [Google Scholar]
  3. Bauer A.W., Kirby W.M.M., Sherris J.C., Turck M. ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45 493496 [PubMed].
    [Google Scholar]
  4. Becker B., Lechevalier M.P., Lechevalier H.A. ( 1965;). Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13 236243 [PubMed].
    [Google Scholar]
  5. Bernardet J.F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 10491070 [View Article] [PubMed].
    [Google Scholar]
  6. Breznak J.A. ( 2004;). Invertebrates – insects. . In Microbial Diversity and Bioprospecting, pp. 191203. Edited by Bull A. T. Washington, DC: American Society for Microbiology; [View Article].
    [Google Scholar]
  7. Broderick N.A., Raffa K.F., Goodman R.M., Handelsman J. ( 2004;). Census of the bacterial community of the gypsy moth larval midgut by using culturing and culture-independent methods. Appl Environ Microbiol 70 293300 [View Article] [PubMed].
    [Google Scholar]
  8. Cai Y., Pang H., Kitahara M., Ohkuma M. ( 2012;). Lactobacillus nasuensis sp. nov., a lactic acid bacterium isolated from silage, and emended description of the genus Lactobacillus. Int J Syst Evol Microbiol 62 11401144 [View Article] [PubMed].
    [Google Scholar]
  9. Claesson M.J., van Sinderen D., O'Toole P.W. ( 2008;). Lactobacillus phylogenomics – towards a reclassification of the genus. Int J Syst Evol Microbiol 58 29452954 [View Article] [PubMed].
    [Google Scholar]
  10. Engel P., Martinson V.G., Moran N.A. ( 2012;). Functional diversity within the simple gut microbiota of the honey bee. Proc Natl Acad Sci U S A 109 1100211007 [View Article] [PubMed].
    [Google Scholar]
  11. Evans J.D., Armstrong T.N. ( 2006;). Antagonistic interactions between honey bee bacterial symbionts and implications for disease. BMC Ecol 6 4 [View Article] [PubMed].
    [Google Scholar]
  12. Evans J.D., Spivak M. ( 2010;). Socialized medicine: individual and communal disease barriers in honey bees. J Invertebr Pathol 103 (Suppl 1) S62S72 [View Article] [PubMed].
    [Google Scholar]
  13. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid – deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [View Article].
    [Google Scholar]
  14. Felsenstein J. ( 1985;). Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  15. Garvie E.I. ( 1986;). Genus Leuconostoc . . In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 10711075. Edited by Sneath P., Mair N., Sharpe M., Holt J. Baltimore, MD: Williams & Wilkins;.
    [Google Scholar]
  16. Hall T.A. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 9598.
    [Google Scholar]
  17. Hammes W.P., Hertel Ch. ( 2009;). Genus Lactobacillus . . In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 465511. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B. New York: Springer;.
    [Google Scholar]
  18. Killer J., Dubná S., Sedláček I., Švec P. ( 2014a;). Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood. Int J Syst Evol Microbiol 64 152157 [View Article] [PubMed].
    [Google Scholar]
  19. Killer J., Švec P., Sedlácek I., Cernohlávková J., Benada O., Hroncová Z., Havlík J., Vlková E., Rada V., other authors. ( 2014b;). Vagococcus entomophilus sp. nov., from the digestive tract of a wasp (Vespula vulgaris). Int J Syst Evol Microbiol 64 731737 [View Article] [PubMed].
    [Google Scholar]
  20. Kim M.K., Im W.T., Ohta H., Lee M., Lee S.T. ( 2005;). Sphingopyxis granuli sp. nov., a β-glucosidase-producing bacterium in the family Sphingomonadaceae in α-4 subclass of the Proteobacteria . J Microbiol 43 152157.
    [Google Scholar]
  21. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  22. Kimura M. ( 1983). The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [View Article].
    [Google Scholar]
  23. Lane D.J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115176. Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;.
    [Google Scholar]
  24. London J. ( 1976;). The ecology and taxonomic status of the lactobacilli. Annu Rev Microbiol 30 279301 [View Article] [PubMed].
    [Google Scholar]
  25. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39 159167 [View Article].
    [Google Scholar]
  26. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  27. Moran N.A., Hansen A.K., Powell J.E., Sabree Z.L. ( 2012;). Distinctive gut microbiota of honey bees assessed using deep sampling from individual worker bees. PLoS One 7 e36393 [View Article] [PubMed].
    [Google Scholar]
  28. Naser S.M., Thompson F.L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. ( 2005;). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151 21412150 [View Article] [PubMed].
    [Google Scholar]
  29. Ohashi Y., Ushida K. ( 2009;). Health-beneficial effects of probiotics: its mode of action. Anim Sci J 80 361371 [View Article] [PubMed].
    [Google Scholar]
  30. Ouwehand A.C., Salminen S., Isolauri E. ( 2002;). Probiotics: an overview of beneficial effects. Antonie van Leeuwenhoek 82 279289 [View Article] [PubMed].
    [Google Scholar]
  31. Patterson J.A., Burkholder K.M. ( 2003;). Application of prebiotics and probiotics in poultry production. Poult Sci 82 627631 [View Article] [PubMed].
    [Google Scholar]
  32. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425.
    [Google Scholar]
  33. Sandine W.E. ( 1979;). Role of Lactobacillus in the intestinal tract. J Food Prot 42 259262.
    [Google Scholar]
  34. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  35. Schillinger U., Lücke F.K. ( 1987;). Identification of lactobacilli from meat and meat products. Food Microbiol 4 199208 [View Article].
    [Google Scholar]
  36. Schleifer K.H., Kandler O. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36 407477.
    [Google Scholar]
  37. Sorokin D.Y. ( 2005;). Is there a limit for high-pH life?. Int J Syst Evol Microbiol 55 14051406 [View Article] [PubMed].
    [Google Scholar]
  38. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed].
    [Google Scholar]
  39. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed].
    [Google Scholar]
  40. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  41. Weiss N., Schillinger U. ( 1984;). Lactobacillus sanfrancisco sp. nov., nom. rev. Syst Appl Microbiol 5 230232 [View Article].
    [Google Scholar]
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