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Volume 65, Issue Pt_10

gen. nov., sp. nov., a mesophilic type I methanotroph isolated from rice rhizosphere Free

Abstract

An aerobic, methane-oxidizing bacterium (strain RS11D-Pr) was isolated from rice rhizosphere. Cells of strain RS11D-Pr were Gram-stain-negative, motile rods with a single polar flagellum and contained an intracytoplasmic membrane system typical of type I methanotrophs. The strain utilized methane and methanol as sole carbon and energy sources. It could grow at 20–37 °C (optimum 31–33 °C), at pH 6.8–7.4 (range 5.5–9.0) and with 0–0.2 % (w/v) NaCl (there was no growth at above 0.5 % NaCl). and genes were present. The ribulose monophosphate and/or ribulose bisphosphate pathways were used for carbon assimilation. Results of sequence analysis of 16S rRNA genes showed that strain RS11D-Pr is related closely to the genera , , and in the family . The similarity was low (94.6 %) between strain RS11D-Pr and the most closely related type strain ( R-49797). The DNA G+C content was 64.1 mol%. Results of phylogenetic analysis of the gene and chemotaxonomic data regarding the major cellular fatty acids (Cω7, C and C) and the major respiratory quinone (MQ-8) also indicated the affiliation of strain RS11D-Pr to the ––– clade. On the basis of phenotypic, genotypic and phylogenetic characteristics, strain RS11D-Pr is considered to represent a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is RS11D-Pr ( = JCM 18894 = NBRC 109438 = DSM 29768 = KCTC 4681).

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Funding
This study was supported by the:
  • Japan Society for the Promotion of Sciences (Award 25292207)
  • ESPEC Foundation for Global Environment Research and Technology (Award 5331)
  • Chubu Science and Technology Center
  • Japan Science and Technology Agency
  • Japan Society for the Promotion of Science
© 2015 IUMS
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2015-10-01
2024-04-24
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Methylomagnum ishizawai gen. nov., sp. nov., a mesophilic type I methanotroph isolated from rice rhizosphere
Int J Syst Evol Microbiol 65, 3527 (2015); https://doi.org/10.1099/ijsem.0.000451
/content/journal/ijsem/10.1099/ijsem.0.000451
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