1887
  1. Home
  2. Publications
  3. International Journal of Systematic and Evolutionary Microbiology
  4. Volume 65, Issue 10
  5. Article

f sp. nov. and sp. nov., two ascosporic yeast species isolated from the gut of beetles

HTML
54.40 Kb
PDF
324.82 Kb
  • Cover date:
Preview this:
Free Preview thumbnail Magnify
Zoom in
Zoomout

sp. nov. and sp. nov., two ascosporic yeast species isolated from the gut of beetles, Page 1 of 1

| /docserver/preview/fulltext/ijsem/65/10/3580_ijsem000460-1.gif

  • Four yeast strains were isolated from the gut of beetles collected on Baotianman Mountain and People's Park of Nanyang in Henan Province, China. These strains produced unconjugated asci with one or two ellipsoidal to elongate ascospores in a persistent ascus. Phylogenetic analysis of the D1/D2 domains of the LSU rRNA gene sequences indicated that the isolates represent two novel sexual species in the / clade. sp. nov. was located in a statistically well-supported branch together with . sp. nov. formed a subclade with its closest relative supported by a strong bootstrap value. The two novel species were distinguished from their most closely related described species, and , in the D1/D2 LSU rRNA gene and internal transcribed spacer (ITS) sequences and in phenotypic traits. The type strain of sp. nov. is NYNU 14719 ( = CBS 13915 = CICC 33052), and the type strain of sp. nov. is NYNU 14772 ( = CBS 13916 = CICC 33053). The MycoBank numbers for sp. nov. and sp. nov. are MB 812621 and MB 812622.

  • The GenBank/EMBL/DDBJ accession numbers for the sequences of the D1/D2 domains of the LSU rRNA gene and ITS regions of strains NYNU 14719 and NYNU 14772 are KM586733–KM586736, respectively.

  • One supplementary figure is available with the online Supplementary Material.

  • Abbreviation:
    ITS internal transcribed spacer

© 2015 IUMS | Published by the Microbiology Society

  • Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J.. ( 1997;). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25: 3389––3402 [CrossRef] [PubMed].
     [Google Scholar]
  • Chang C.F., Lin Y.C., Chen S.F., Carvajal Barriga E.J., Barahona P.P., James S.A., Bond C.J., Roberts I.N., Lee C.F.. ( 2012;). Candida theae sp. nov., a new anamorphic beverage-associated member of the Lodderomyces clade. Int J Food Microbiol 153: 10––14 [CrossRef] [PubMed].
     [Google Scholar]
  • Daniel H.-M., Lachance M.-A., Kurtzman C.P.. ( 2014;). On the reclassification of species assigned to Candida and other anamorphic ascomycetous yeast genera based on phylogenetic circumscription. Antonie van Leeuwenhoek 106: 67––84 [CrossRef] [PubMed].
     [Google Scholar]
  • Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783––791 [CrossRef].
     [Google Scholar]
  • Hawksworth D.L.. ( 2011;). A new dawn for the naming of fungi: impacts of decisions made in Melbourne in July 2011 on the future publication and regulation of fungal names. IMA Fungus 2: 155––162 [CrossRef] [PubMed].
     [Google Scholar]
  • Hui F.L., Niu Q.H., Ke T., Li Y.X., Lee C.F.. ( 2013;). Candida sanyaensis sp. nov., an ascomycetous yeast species isolated from soil. Antonie van Leeuwenhoek 103: 47––52 [CrossRef] [PubMed].
     [Google Scholar]
  • Ji Z.H., Jia J.H., Bai F.Y.. ( 2009;). Four novel Candida species in the Candida albicans/Lodderomyces elongisporus clade isolated from the gut of flower beetles. Antonie van Leeuwenhoek 95: 23––32 [CrossRef] [PubMed].
     [Google Scholar]
  • Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111––120 [CrossRef] [PubMed].
     [Google Scholar]
  • Kurtzman C.P.. ( 2011;). Lodderomyces van der Walt (1971). . In The Yeasts, a Taxonomic Study, 2, , 5th edn.., pp. 561––563. Edited by Kurtzman C. P., Fell J. W., Boekhout T.. Amsterdam: Elsevier; [CrossRef].
     [Google Scholar]
  • Kurtzman C.P., Robnett C.J.. ( 1998;). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73: 331––371 [CrossRef] [PubMed].
     [Google Scholar]
  • Kurtzman C.P., Suzuki M.. ( 2010;). Phylogenetic analysis of ascomycetous yeasts that form coenzyme Q-9 and the proposal of the new genera Babjeviella, Meyerozyma, Millerozyma, Priceomyces, and Scheffersomyces. Mycoscience 51: 2––14 [CrossRef].
     [Google Scholar]
  • Kurtzman C.P., Fell J.W., Boekhout T., Robert V.. ( 2011;). Methods for isolation, phenotypic characterization and maintenance of yeasts. . In The Yeasts, a Taxonomic Study, 1, , 5th edn.., pp. 87––110. Edited by Kurtzman C. P., Fell J. W., Boekhout T.. Amsterdam: Elsevier; [CrossRef].
     [Google Scholar]
  • Lachance M.A., Boekhout T., Scorzetti G., Fell J.W., Kurtzman C.P.. ( 2011;). Candida Berkhout (1923). . In The Yeasts, a Taxonomic Study, 2, , 5th edn.., pp. 987––1278. Edited by Kurtzman C. P., Fell J. W., Boekhout T.. Amsterdam: Elsevier; [CrossRef].
     [Google Scholar]
  • Nguyen N.H., Suh S.-O., Blackwell M.. ( 2007;). Five novel Candida species in insect-associated yeast clades isolated from Neuroptera and other insects. Mycologia 99: 842––858 [CrossRef] [PubMed].
     [Google Scholar]
  • Nitiyon S., Boonmak C., Am-In S., Jindamorakot S., Kawasaki H., Yongmanitchai W., Limtong S.. ( 2011;). Candida saraburiensis sp. nov. and Candida prachuapensis sp. nov., xylose-utilizing yeast species isolated in Thailand. Int J Syst Evol Microbiol 61: 462––468 [CrossRef] [PubMed].
     [Google Scholar]
  • Suh S.-O., Nguyen N.H., Blackwell M.. ( 2008;). Yeasts isolated from plant-associated beetles and other insects: seven novel Candida species near Candida albicans. FEMS Yeast Res 8: 88––102 [CrossRef] [PubMed].
     [Google Scholar]
  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731––2739 [CrossRef] [PubMed].
     [Google Scholar]
  • Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876––4882 [CrossRef] [PubMed].
     [Google Scholar]
  • Urbina H., Schuster J., Blackwell M.. ( 2013;). The gut of Guatemalan passalid beetles: a habitat colonized by cellobiose- and xylose-fermenting yeasts. Fungal Ecol 6: 339––355 [CrossRef].
     [Google Scholar]
  • White T.J., Bruns T., Lee S., Taylor J.. ( 1990;). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications, pp. 315––322. Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J.. San Diego, CA: Academic Press; [CrossRef].
     [Google Scholar]
http://sgm.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000460
Loading

Supplementary Data

 Data loading....

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000460
2015-10-01
2019-02-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/10/3580.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000460&mimeType=html&fmt=ahah

Author and Article Information

 Figure data loading....

Footnotes:

Validated antibodies in this article

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error