1887

Abstract

Two independent isolates of a Gram-stain-positive, facultatively anaerobic, motile, rod-shaped bacterium were recovered from cheonggukjang, a Korean fermented soybean paste food product. Preliminary sequencing analysis of the 16S rRNA gene indicated that these strains were related most closely to KCTC-13918 and DSM 13. In phenotypic characterization, the novel strains were found to grow between 15 and 55 °C and to tolerate up to 8 % (w/v) NaCl. Furthermore, the strains grew in media of pH 5–10 (optimal growth at pH 7.0). The predominant cellular fatty acids were anteiso-C and iso-C.The isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained -diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown glycolipid. Draft genomes of the two strains were determined and DNA–DNA hybridizations with their nearest neighbour ( KCTC-13918) revealed 29.9 % relatedness for both strains. Phylogenomic analysis of the genomes was conducted with the core genome (799 genes) of all strains in the group and the two strains formed a distinct monophyletic cluster. In addition, the strains differed from the two most closely related species in that they did not metabolize maltose, -galactose, -sorbitol or -gluconic acid. The DNA G+C content was 45.9 mol%. Based upon the consensus of phylogenetic and phenotypic analyses, these strains represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GO-13 ( = KACC 18425 = NRRL B-65291).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000462
2015-10-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/10/3586.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000462&mimeType=html&fmt=ahah

References

  1. Auch A.F., von Jan M., Klenk H.P., Göker M. ( 2010;). Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2 117134 [View Article] [PubMed].
    [Google Scholar]
  2. Breznak J.A., Costilow R.N. ( 1994;). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  3. Dunlap C. ( 2015;). 10.1099 The status of the species Bacillus aerius. Request for an Opinion. Int J Syst Evol Microbiol 65.
    [Google Scholar]
  4. Jolley K.A., Maiden M.C. ( 2010;). BIGSdb: Scalable analysis of bacterial genome variation at the population level. BMC Bioinformatics 11 595 [View Article] [PubMed].
    [Google Scholar]
  5. Jung J.B., Choi S.K., Jeong D.Y., Kim Y.S., Kim Y.-S. ( 2012;). Effects of germination time of soybeans on quality characteristics of cheonggukjang fermented with an isolated bacterial strain. Korean J Food Sci Techn 44 6975 [View Article].
    [Google Scholar]
  6. Logan N.A., De Vos P. ( 2009;). Family I. Bacillaceae . . In Bergey's Manual of Systematic Bacteriology. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. , 2nd edn.. Dordrect: Springer;.
    [Google Scholar]
  7. Meier-Kolthoff J.P., Auch A.F., Klenk H.P., Göker M. ( 2013;). Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14 60 [View Article] [PubMed].
    [Google Scholar]
  8. Overbeek R., Olson R., Pusch G.D., Olsen G.J., Davis J.J., Disz T., Edwards R.A., Gerdes S., Parrello B., other authors. ( 2014;). The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res 42 (D1), D206D214 [View Article] [PubMed].
    [Google Scholar]
  9. Palmisano M.M., Nakamura L.K., Duncan K.E., Istock C.A., Cohan F.M. ( 2001;). Bacillus sonorensis sp. nov., a close relative of Bacillus licheniformis, isolated from soil in the Sonoran Desert, Arizona. Int J Syst Evol Microbiol 51 16711679 [View Article] [PubMed].
    [Google Scholar]
  10. Staneck J.L., Roberts G.D. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28 226231 [PubMed].
    [Google Scholar]
  11. Sumpavapol P., Tongyonk L., Tanasupawat S., Chokesajjawatee N., Luxananil P., Visessanguan W. ( 2010;). Bacillus siamensis sp. nov., isolated from salted crab (poo-khem) in Thailand. Int J Syst Evol Microbiol 60 23642370 [View Article] [PubMed].
    [Google Scholar]
  12. Tamura K., Nei M. ( 1993;). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10 512526 [PubMed].
    [Google Scholar]
  13. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed].
    [Google Scholar]
  14. Tindall B.J. ( 1990;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13 128130 [View Article].
    [Google Scholar]
  15. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000462
Loading
/content/journal/ijsem/10.1099/ijsem.0.000462
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error