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f Taxonomic description and genome sequence of sp. nov., a halotolerant bacterium isolated from marine sediment

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Taxonomic description and genome sequence of sp. nov., a halotolerant bacterium isolated from marine sediment, Page 1 of 1

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  • A Gram-staining-positive, coccoid, halotolerant bacterial strain, designated SV-16, was isolated from marine sediment and subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus . Growth occurred at temperatures in the range 25–37 °C (optimum 30 °C), at pH 7.0–11.0 (optimum pH 8.0) and at NaCl concentrations of up to 25.0 % (optimum 15.0 %). The highest level of 16S rRNA gene sequence similarity was with Crm (98.6 %) followed by W24 (96.6 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylglycerol. The major cellular fatty acids were iso-C, anteiso-C, iso-C and anteiso-C. The draft genome of strain SV-16 consisted of 2 591 284 bp with a DNA G+C content of 48.7 mol%. On the basis of the phenotypic characteristics and genotypic distinctiveness of strain SV-16, it should be classified within a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SV-16 ( = MTCC 11832 = DSM 28797).

  • These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SV-16 is KF701618. This whole genome shotgun project has been deposited under accession number LAYZ00000000. The version described in this paper is LAYZ01000000.

  • One supplementary figure is available with the online Supplementary Material.

  • Abbreviations:
    ANI average nucleotide identity
    DDDH digital DNA–DNA hybridization

© 2015 IUMS | Published by the Microbiology Society

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2015-11-01
2019-02-19
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