RT Journal Article SR Electronic(1) A1 Niu, Lili A1 Tang, Tianyi A1 Ma, Zhongliang A1 Song, Lei A1 Zhang, Kegui A1 Chen, Yuanyuan A1 Hua, Ziyi A1 Hu, Xing A1 Zhao, MengYR 2015 T1 Paenibacillus yunnanensis sp. nov., isolated from Pu'er tea JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_11 SP 3806 OP 3811 DO https://doi.org/10.1099/ijsem.0.000496 PB Microbiology Society, SN 1466-5034, AB A novel Gram-staining-positive, aerobic, endospore-forming, rod-shaped bacterial strain, YN2T, was isolated from ripened Pu'er tea. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain represented a novel species of the genus Paenibacillus. The strains most closely related to strain YN2T were Paenibacillus vulneris JCM 18268T and Paenibacillus rigui JCM 16352T, with 16S rRNA similarities of 98.6 and 95.5 %, respectively. Chemotaxonomic data supported the affiliation of the new isolate to the genus Paenibacillus, including MK-7 as the major menaquinone, DNA G+C content of 51 mol%, cell-wall type A1γ (meso-diaminopimelic acid as the diagnostic diamino acid) and anteiso-C15 : 0 and iso-C16 : 0 as the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine and phospholipid. Strain YN2T could be differentiated from recognized species of the genus Paenibacillus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of evidence from this polyphasic study, Paenibacillus yunnanensis sp. nov., is proposed, with strain YN2T ( = CGMCC 1.12968T = JCM 30953T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000496