@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000501, author = "Shimane, Yasuhiro and Minegishi, Hiroaki and Echigo, Akinobu and Kamekura, Masahiro and Itoh, Takashi and Ohkuma, Moriya and Tsubouchi, Taishi and Usui, Keiko and Maruyama, Tadashi and Usami, Ron and Hatada, Yuji", title = "Halarchaeum grantii sp. nov., a moderately acidophilic haloarchaeon isolated from a commercial salt sample", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2015", volume = "65", number = "Pt_11", pages = "3830-3835", doi = "https://doi.org/10.1099/ijsem.0.000501", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000501", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Three moderately acidophilic, halophilic archaeal strains, MH1-243-3T, MH1-243-5 and MH1-243-6, were isolated from a commercial salt sample made from seawater in Okinawa, Japan. Cells of the three strains were pleomorphic and stained Gram-negative. Colonies of the strains were orange–red-pigmented. Strain MH1-243-3T was able to grow at 15–27 % (w/v) NaCl (optimum 24 °C), at pH 4.5–6.5 (pH 5.5) and at 35–50 °C (45 °C). Strains MH1-243-5 and MH1-243-6 grew within slightly different ranges (shown in text). The 16S rRNA gene sequences of the three strains were identical, and the closest phylogenetic relative was Halarchaeum salinum MH1-34-1T with 97.0 % similarity. The rpoB′ gene sequences of the three strains were also identical, and the closest phylogenetic relative was Halarchaeum acidiphilum JCM 16109T with 92.0 % similarity. The DNA G+C content of MH1-243-3T, MH1-243-5 and MH1-243-6 was 65.2 mol%. The levels of DNA–DNA relatedness amongst the three strains were 84.1–99.8 %, while that between MH1-243-3T and H. salinum MH1-34-1T was 30.6 % and 31.6 % (reciprocally), and those between MH1-243-3T and type strains of other species in the genus Halarchaeum were 42.3–29.4 %. Based on the phenotypic, genotypic and phylogenetic analyses, it is proposed that the isolates should represent a novel species of the genus Halarchaeum, for which the name Halarchaeum grantii sp. nov. is proposed. The type strain is MH1-243-3T ( = JCM 19585T = KCTC 4142T), isolated from commercial sea salt produced in Okinawa, Japan. MH1-243-5 ( = JCM 19586) and MH1-243-6 ( = JCM 18422) are additional strains of the species.", }