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f gen. nov., sp. nov., isolated from seawater

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  • A novel Gram-stain-negative, rod-shaped, yellow-pigmented, non-sporulating, non-motile bacterium, designated strain AK20, was isolated from seawater collected from Kochi city, Kerala state, India. Colonies on marine agar were circular, yellow, shiny, translucent, 2–3 mm in diameter, convex and with entire margin. Flexirubin-type pigment was present. The fatty acids were dominated by iso-branched units with a high abundance of iso-C, iso-C G, iso-C 3-OH, summed feature 3 (Cω7 and/or iso-C 2-OH) and iso-C 3-OH. Polar lipids included phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and four unidentified lipids. Menaquinone 6 (MK-6) was the predominant respiratory quinone. The DNA G+C content of strain AK20 was 38.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AK20 was closely related to A2 and KMM 6029 (pair-wise sequence similarities of 95.9 and 95.7 %, respectively), forming a distinct branch within the family and clustering with the clade comprising species of the genus . Based on phenotypic and chemotaxonomic characteristics and phylogenetic analysis, strain AK20 is different from the existing genera in the family , and is therefore considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is AK20 ( = MTCC 11705 = JCM 18821).

  • All authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain AK20 is HE653972.

  • One supplementary table and two supplementary figures are available with the online Supplementary Material.

© 2015 IUMS | Published by the Microbiology Society

  • Anil Kumar P., Srinivas T. N. R., Madhu S., Sravan R., Singh S., Naqvi S. W. A., Mayilraj S., Shivaji S.. ( 2012;). Cecembia lonarensis gen. nov., sp. nov., a haloalkalitolerant bacterium of the family Cyclobacteriaceae, isolated from a haloalkaline lake and emended descriptions of the genera Indibacter, Nitritalea and Belliella. Int J Syst Evol Microbiol 62: 2252––2258 [CrossRef] [PubMed].
     [Google Scholar]
  • Arun A. B., Chen W. M., Lai W. A., Chou J. H., Shen F. T., Rekha P. D., Young C. C.. ( 2009;). Lutaonella thermophila gen. nov., sp. nov., a moderately thermophilic member of the family Flavobacteriaceae isolated from a coastal hot spring. Int J Syst Evol Microbiol 59: 2069––2073 [CrossRef] [PubMed].
     [Google Scholar]
  • Bernardet J.-F., Nakagawa Y.. ( 2006;). An introduction to the family Flavobacteriaceae. . In The Prokaryotes: a Handbook on the Biology of Bacteria, pp. 455––480. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E..vol. 7, 3rd edn.., New York: Springer; [CrossRef].
     [Google Scholar]
  • Bernardet J.-F., Nakagawa Y., Holmes B.. ( 2002;). Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049––1070 [CrossRef] [PubMed].
     [Google Scholar]
  • Bligh E. G., Dyer W. J.. ( 1959;). A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911––917 [CrossRef] [PubMed].
     [Google Scholar]
  • Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100: 221––230 [CrossRef] [PubMed].
     [Google Scholar]
  • Cowan S. T., Steel K. J.. ( 1965;). Manual for the Identification of Medical Bacteria., London: Cambridge University Press;.
     [Google Scholar]
  • Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N.. ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24: 54––63 [CrossRef].
     [Google Scholar]
  • Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K.. ( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47: 1129––1133 [CrossRef] [PubMed].
     [Google Scholar]
  • Haber M., Shefer S., Giordano A., Orlando P., Gambacorta A., Ilan M.. ( 2013;). Aureivirga marina gen. nov., sp. nov., a marine bacterium isolated from the Mediterranean sponge Axinella verrucosa. Int J Syst Evol Microbiol 63: 1089––1095 [CrossRef] [PubMed].
     [Google Scholar]
  • Hameed A., Shahina M., Lai W. A., Lin S. Y., Liu Y. C., Hsu Y. H., Young C. C.. ( 2015;). Hanstruepera neustonica gen. nov., sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from estuarine water, and emendation of Sediminibacter furfurosus Khan et al. 2007 emend. Kwon et al. 2014, Mangrovimonas yunxiaonensis Li et al. 2013, Antarcticimonas flava Yang et al. 2009 and Hoppeia youngheungensis Kwon et al. 2014. Int J Syst Evol Microbiol 65: 336––345 [CrossRef] [PubMed].
     [Google Scholar]
  • Ivanova E. P., Alexeeva Y. V., Flavier S., Wright J. P., Zhukova N. V., Gorshkova N. M., Mikhailov V. V., Nicolau D. V., Christen R.. ( 2004;). Formosa algae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae. Int J Syst Evol Microbiol 54: 705––711 [CrossRef] [PubMed].
     [Google Scholar]
  • Jeong S. H., Park M. S., Jin H. M., Lee K., Park W., Jeon C. O.. ( 2013;). Aestuariibaculum suncheonense gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a tidal flat and emended descriptions of the genera Gaetbulibacter and Tamlana. Int J Syst Evol Microbiol 63: 332––338 [CrossRef] [PubMed].
     [Google Scholar]
  • Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21––132. Edited by Munro H. N.. New York: Academic Press; [CrossRef].
     [Google Scholar]
  • Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716––721 [CrossRef] [PubMed].
     [Google Scholar]
  • Komagata K., Suzuki K.. ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19: 161––207 [CrossRef].
     [Google Scholar]
  • Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115––175. Edited by Stackebrandt E., Goodfellow M.. Chichester: Wiley;.
     [Google Scholar]
  • Lányí B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19: 1––67 [CrossRef].
     [Google Scholar]
  • Lee D. H., Cho S. J., Kim S. M., Lee S. B.. ( 2013;). Psychroserpens damuponensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63: 703––708 [CrossRef] [PubMed].
     [Google Scholar]
  • Li Y., Bai S., Yang C., Lai Q., Zhang H., Chen Z., Wei J., Zheng W., Tian Y., Zheng T.. ( 2013;). Mangrovimonas yunxiaonensis gen. nov., sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 63: 2043––2048 [CrossRef] [PubMed].
     [Google Scholar]
  • Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., other authors. ( 2004;). arb: a software environment for sequence data. Nucleic Acids Res 32: 1363––1371 [CrossRef] [PubMed].
     [Google Scholar]
  • Nakajima K., Hirota K., Nodasaka Y., Yumoto I.. ( 2005;). Alkalibacterium iburiense sp. nov., an obligate alkaliphile that reduces an indigo dye. Int J Syst Evol Microbiol 55: 1525––1530 [CrossRef] [PubMed].
     [Google Scholar]
  • Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Bae K. S.. ( 2005;). Bizionia paragorgiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea. Int J Syst Evol Microbiol 55: 375––378 [CrossRef] [PubMed].
     [Google Scholar]
  • Nedashkovskaya O. I., Suzuki M., Lee J. S., Lee K. C., Shevchenko L. S., Mikhailov V. V.. ( 2009;). Pseudozobellia thermophila gen. nov., sp. nov., a bacterium of the family Flavobacteriaceae, isolated from the green alga Ulva fenestrata. Int J Syst Evol Microbiol 59: 806––810 [CrossRef] [PubMed].
     [Google Scholar]
  • Sly L. I., Blackall L. L., Kraat P. C., Tao T. S., Sangkhobol V.. ( 1986;). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5: 139––156 [CrossRef].
     [Google Scholar]
  • Surendra V., Bhawana P., Suresh K., Srinivas T. N. R., Anil Kumar P.. ( 2012;). Imtechella halotolerans gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from estuarine water. Int J Syst Evol Microbiol 62: 2624––2630 [CrossRef] [PubMed].
     [Google Scholar]
  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731––2739 [CrossRef] [PubMed].
     [Google Scholar]
  • Wu Y. H., Yu P. S., Zhou Y. D., Xu L., Wang C. S., Wu M., Oren A., Xu X. W.. ( 2013;). Muricauda antarctica sp. nov., a marine member of the Flavobacteriaceae isolated from Antarctic seawater. Int J Syst Evol Microbiol 63: 3451––3456 [CrossRef] [PubMed].
     [Google Scholar]
  • Xu Y., Tian X. P., Liu Y. J., Li J., Kim C. J., Yin H., Li W.-J., Zhang S.. ( 2013;). Sinomicrobium oceani gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment. Int J Syst Evol Microbiol 63: 1045––1050 [CrossRef] [PubMed].
     [Google Scholar]
  • Yassin A. F., Inglis T. J. J., Hupfer H., Siering C., Schumann P., Busse H. J., Aravena-Román M.. ( 2012;). Cruoricaptor ignavus gen. nov., sp. nov., a novel bacterium of the family Flavobacteriaceae isolated from blood culture of a man with bacteraemia. Syst Appl Microbiol 35: 421––426 [CrossRef] [PubMed].
     [Google Scholar]
  • Yoon J., Jang J. H., Kasai H.. ( 2013;). Spongiimonas flava gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from an unidentified marine sponge. Antonie van Leeuwenhoek 103: 625––633 [CrossRef] [PubMed].
     [Google Scholar]
  • Zhang X. Y., Liu A., Liu C., Li H., Li G. W., Xu Z., Chen X. L., Zhou B. C., Zhang Y. Z.. ( 2013;). Arenitalea lutea gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from intertidal sand. Int J Syst Evol Microbiol 63: 2853––2858 [CrossRef] [PubMed].
     [Google Scholar]
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2015-11-01
2019-02-16
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