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Volume 65, Issue Pt_11

gen. nov., sp. nov., isolated from marine sediment Free

Abstract

A Gram-staining-positive, strictly aerobic, spore-forming and rod-shaped motile bacterium with peritrichous flagellae, designated strain S1203, was isolated from the sediment of the northern Okinawa Trough. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S1203 formed a lineage within the family that was distinct from the most closely related genera , , and with gene sequence similarities ranging from 86.2 to 93.76 %. Optimal growth occurred in the presence of 4–8 % (w/v) NaCl, at pH 7.0–8.0 and 25–32 °C. The cell-wall peptidoglycan was based on diaminopimelic acid and unsaturated menaquinone with seven isoprene units (MK-7) as the predominant respiratory quinone. The major fatty acids (>10 % of total fatty acids) were anteiso-C, iso-C and C.The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and an unidentified phospholipid. The DNA G+C content of strain S1203 was 47.7 mol%. On the basis of polyphasic analysis, strainS1203 was considered to represent a novel species in a new genus of the family , for which the name gen. nov., sp. nov. is proposed; the type strain of is S1203 ( = DSM 28697 = JCM 30067 = MCCC 1K00259).

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Funding
This study was supported by the:
  • the Major State Basic Research Development Program of China (Award 2013CB429700)
  • the China Ocean Mineral Resources Research & Development Association (Award DY125-15-R-03)
  • the National High Technology Research & Development Program of China (Award 2012AA091605)
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2015-11-01
2024-04-25
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References

  1. Albert R. A., Archambault J., Rosselló-Mora R., Tindall B. J., Matheny M. ( 2005;). Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55 2125–2130 [View Article] [PubMed].
     [Google Scholar]
  2. Albuquerque L., Tiago I., Taborda M., Nobre M. F., Veríssimo A., da Costa M. S. ( 2008;). Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond. Int J Syst Evol Microbiol 58 226–230 [View Article] [PubMed].
     [Google Scholar]
  3. Arahal D. R., Márquez M. C., Volcani B. E., Schleifer K. H., Ventosa A. ( 2000;). Reclassification of Bacillus marismortui as Salibacillus marismortui comb. nov. Int J Syst Evol Microbiol 50 1501–1503. [CrossRef]
    [Google Scholar]
  4. Arfman N., Dijkhuizen L., Kirchhof G., Ludwig W., Schleifer K. H., Bulygina E. S., Chumakov K. M., Govorukhina N. I., Trotsenko Y. A., other authors. ( 1992;). Bacillus methanolicus sp. nov., a new species of thermotolerant, methanol-utilizing, endospore-forming bacteria. Int J Syst Bacteriol 42 439–445 [View Article] [PubMed].
     [Google Scholar]
  5. Beveridge T., Lawrence J., Murray R. ( 2007;). Sampling and staining for light microscopy. . In Methods for General and Molecular Microbiology, pp. 19–33. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  6. Chen Y. G., Cui X. L., Wang Y. X., Zhang Y. Q., Tang S. K., Li W. J., Liu Z. X., Wen M. L., Peng Q. ( 2009;). Virgibacillus sediminis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 59 2058–2063 [View Article] [PubMed].
     [Google Scholar]
  7. Claus D., Berkeley R. C. W. ( 1986;). Genus Bacillus Cohn 1872. . In Bergey's Manual of Systematic Bacteriology, pp. 1105–1139. Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. 2 Baltimore: Williams & Wilkins;.
     [Google Scholar]
  8. Dai X., Wang Y. N., Wang B. J., Liu S. J., Zhou Y. G. ( 2005;). Planomicrobium chinense sp. nov., isolated from coastal sediment, and transfer of Planococcus psychrophilus and Planococcus alkanoclasticus to Planomicrobium as Planomicrobium psychrophilum comb. nov. and Planomicrobium alkanoclasticum comb. nov. Int J Syst Evol Microbiol 55 699–702 [View Article] [PubMed].
     [Google Scholar]
  9. Denariaz G., Payne W. J., Le Gall J. ( 1989;). A halophilic denitrifier, Bacillus halodenitrificans sp. nov. Int J Syst Bacteriol 39 145–151 [View Article].
     [Google Scholar]
  10. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376 [View Article] [PubMed].
     [Google Scholar]
  11. Garabito M. J., Márquez M. C., Ventosa A. ( 1998;). Halotolerant Bacillus diversity in hypersaline environments. Can J Microbiol 44 95–102 [View Article].
     [Google Scholar]
  12. Heyndrickx M., Lebbe L., Kersters K., De Vos P., Forsyth G., Logan N. A. ( 1998;). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 48 99–106 [View Article].
     [Google Scholar]
  13. Heyndrickx M., Lebbe L., Kersters K., Hoste B., De Wachter R., De Vos P., Forsyth G., Logan N. A. ( 1999;). Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998. Int J Syst Bacteriol 49 1083–1090 [View Article] [PubMed].
     [Google Scholar]
  14. Heyrman J., Logan N. A., Busse H. J., Balcaen A., Lebbe L., Rodriguez-Diaz M., Swings J., De Vos P. ( 2003;). Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus . Int J Syst Evol Microbiol 53 501–511 [View Article] [PubMed].
     [Google Scholar]
  15. Heyrman J., Vanparys B., Logan N. A., Balcaen A., Rodríguez-Díaz M., Felske A., De Vos P. ( 2004;). Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. Int J Syst Evol Microbiol 54 47–57 [View Article] [PubMed].
     [Google Scholar]
  16. Hua N. P., Kanekiyo A., Fujikura K., Yasuda H., Naganuma T. ( 2007;). Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. Int J Syst Evol Microbiol 57 1243–1249 [View Article] [PubMed].
     [Google Scholar]
  17. Kämpfer P., Falsen E., Lodders N., Langer S., Busse H. J., Schumann P. ( 2010;). Ornithinibacillus contaminans sp. nov., an endospore-forming species. Int J Syst Evol Microbiol 60 2930–2934 [View Article] [PubMed].
     [Google Scholar]
  18. Kanso S., Greene A. C., Patel B. K. ( 2002;). Bacillus subterraneus sp. nov., an iron- and manganese-reducing bacterium from a deep subsurface Australian thermal aquifer. Int J Syst Evol Microbiol 52 869–874 [View Article] [PubMed].
     [Google Scholar]
  19. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  20. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111–120 [View Article] [PubMed].
     [Google Scholar]
  21. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161–207. [CrossRef]
    [Google Scholar]
  22. Liu B., Gao Y. C., Lin Y. Z., Zhu Y. J., Lan J. L., Zhou H. T., Lu P. ( 2006). Literatures Research of Bacillus Genus., 1-1679., Guangzhou: Guangdong Travel & Tourism Press;.
     [Google Scholar]
  23. Logan N. A., Lebbe L., Verhelst A., Goris J., Forsyth G., Rodríguez-Díaz M., Heyndrickx M., De Vos P. ( 2004;). Bacillus shackletonii sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago. Int J Syst Evol Microbiol 54 373–376 [View Article] [PubMed].
     [Google Scholar]
  24. Lu J., Nogi Y., Takami H. ( 2001;). Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205 291–297 [View Article] [PubMed].
     [Google Scholar]
  25. Lyman J., Fleming R. H. ( 1940;). Composition of sea water. J Mar Res 3 134–146.
     [Google Scholar]
  26. Manorama R., Pindi P. K., Reddy G. S., Shivaji S. ( 2009;). Bhargavaea cecembensis gen. nov., sp. nov., isolated from the Chagos-Laccadive ridge system in the Indian Ocean. Int J Syst Evol Microbiol 59 2618–2623 [View Article] [PubMed].
     [Google Scholar]
  27. Margesin R., Schinner F. ( 2001;). Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5 73–83 [View Article] [PubMed].
     [Google Scholar]
  28. Mayr R., Busse H. J., Worliczek H. L., Ehling-Schulz M., Scherer S. ( 2006;). Ornithinibacillus gen. nov., with the species Ornithinibacillus bavariensis sp. nov. and Ornithinibacillus californiensis sp. nov. Int J Syst Evol Microbiol 56 1383–1389 [View Article] [PubMed].
     [Google Scholar]
  29. Mesbah M., Premachandran U., Whitman W. B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159–167 [View Article].
     [Google Scholar]
  30. Minnikin D., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241 [View Article].
     [Google Scholar]
  31. Montero-Calasanz M., del C., Göker M., Rohde M., Spröer C., Schumann P., Busse H. J., Schmid M., Tindall B. J., Klenk H. P., Camacho M. ( 2013;). Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium . Int J Syst Evol Microbiol 63 4386–4395 [View Article] [PubMed].
     [Google Scholar]
  32. Moore E. R. B., Arnscheidt A., Krüger A., Strömpl C., Mau M. ( 1999;). Simplified protocols for the preparation of genomic DNA from bacterial cultures. . In Microbial Ecology Manual 1.6.1, pp. 1–15. Edited by Akkermans A. D. L., van Elsas J. D., de Bruijn F. J. Dordrecht: Kluwer;.
     [Google Scholar]
  33. Nazina T. N., Tourova T. P., Poltaraus A. B., Novikova E. V., Grigoryan A. A., Ivanova A. E., Lysenko A. M., Petrunyaka V. V., Osipov G. A., other authors. ( 2001;). Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans . Int J Syst Evol Microbiol 51 433–446. [CrossRef]
    [Google Scholar]
  34. Qiu F., Zhang X., Liu L., Sun L., Schumann P., Song W. ( 2009;). Bacillus beijingensis sp. nov. and Bacillus ginsengi sp. nov., isolated from ginseng root. Int J Syst Evol Microbiol 59 729–734 [View Article] [PubMed].
     [Google Scholar]
  35. Rüger H. J. ( 1983;). Differentiation of Bacillus globisporus, Bacillus marinus comb.nov., Bacillus aminovorans and Bacillus insolitus . Int J Syst Bacteriol 33 157–161 [View Article].
     [Google Scholar]
  36. Rüger H. J., Richter G. ( 1979;). Bacillus globisporus subsp. marinus subsp. nov. Int J Syst Bacteriol 29 196–203 [View Article].
     [Google Scholar]
  37. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  38. Schumann P. ( 2011;). Peptidoglycan structure. . In Taxonomy of Prokaryotes (Methods in Microbiology series vol. 38), pp. 101–129. Edited by Rainey F., Oren A. . [CrossRef]
    [Google Scholar]
  39. Seiler H., Wenning M., Schmidt V., Scherer S. ( 2013;). Bacillus gottheilii sp. nov., isolated from a pharmaceutical manufacturing site. Int J Syst Evol Microbiol 63 867–872 [View Article] [PubMed].
     [Google Scholar]
  40. Tamura K., Nei M. ( 1993;). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10 512–526 [PubMed].
     [Google Scholar]
  41. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed].
     [Google Scholar]
  42. Teather R. M., Wood P. J. ( 1982;). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43 777–780 [PubMed].
     [Google Scholar]
  43. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997;). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882 [View Article] [PubMed].
     [Google Scholar]
  44. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. , 3rd edn.., Washington, DC:: [View Article] American Society for Microbiology;.
     [Google Scholar]
  45. Verma P., Pandey P. K., Gupta A. K., Seong C. N., Park S. C., Choe H. N., Baik K. S., Patole M. S., Shouche Y. S. ( 2012;). Reclassification of Bacillus beijingensis Qiu et al. 2009 and Bacillus ginsengi Qiu et al. 2009 as Bhargavaea beijingensis comb. nov. and Bhargavaea ginsengi comb. nov. and emended description of the genus Bhargavaea . Int J Syst Evol Microbiol 62 2495–2504 [View Article] [PubMed].
     [Google Scholar]
  46. Verma P., Seong C. N., Pandey P. K., Bhonde R. R., Spröer C., Rohde M., Shouche Y. S. ( 2013;). Bhargavaea indica sp. nov., a member of the phylum Firmicutes, isolated from Arabian Sea sediment. J Microbiol 51 36–42 [View Article] [PubMed].
     [Google Scholar]
  47. Wainø M., Tindall B. J., Schumann P., Ingvorsen K. ( 1999;). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49 821–831 [View Article] [PubMed].
     [Google Scholar]
  48. Xie C.-H., Yokota A. ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49 345–349 [View Article] [PubMed].
     [Google Scholar]
  49. Yin X., Yang Y., Wang S., Zhang G. ( 2015;). Virgibacillus oceani sp. nov. isolated from ocean sediment. Int J Syst Evol Microbiol 65 159–164 [View Article] [PubMed].
     [Google Scholar]
  50. Yoon J. H., Kang S. S., Lee K. C., Lee E. S., Kho Y. H., Kang K. H., Park Y. H. ( 2001;). Planomicrobium koreense gen. nov., sp. nov., a bacterium isolated from the Korean traditional fermented seafood jeotgal, and transfer of Planococcus okeanokoites (Nakagawa et al. 1996) and Planococcus mcmeekinii (Junge et al. 1998) to the genus Planomicrobium . Int J Syst Evol Microbiol 51 1511–1520 [PubMed]. [CrossRef]
    [Google Scholar]
  51. Yoon J. H., Kim I. G., Kang K. H., Oh T. K., Park Y. H. ( 2004a;). Bacillus hwajinpoensis sp. nov. and an unnamed Bacillus genomospecies, novel members of Bacillus rRNA group 6 isolated from sea water of the East Sea and the Yellow Sea in Korea. Int J Syst Evol Microbiol 54 803–808 [View Article] [PubMed].
     [Google Scholar]
  52. Yoon J. H., Kim I. G., Schumann P., Oh T. K., Park Y. H. ( 2004b;). Marinibacillus campisalis sp. nov., a moderate halophile isolated from a marine solar saltern in Korea, with emended description of the genus Marinibacillus . Int J Syst Evol Microbiol 54 1317–1321 [View Article] [PubMed].
     [Google Scholar]
  53. Yoon J. H., Oh T. K., Park Y. H. ( 2004c;). Transfer of Bacillus halodenitrificans Denariaz et al. 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov. Int J Syst Evol Microbiol 54 2163–2167 [View Article] [PubMed].
     [Google Scholar]
  54. Yoon J. H., Kang S. J., Schumann P., Oh T. K. ( 2010;). Jeotgalibacillus salarius sp. nov., isolated from a marine saltern, and reclassification of Marinibacillus marinus and Marinibacillus campisalis as Jeotgalibacillus marinus comb. nov. and Jeotgalibacillus campisalis comb. nov., respectively. Int J Syst Evol Microbiol 60 15–20 [View Article] [PubMed].
     [Google Scholar]
  55. Yu T., Yin Q., Song X., Zhao R., Shi X., Zhang X.-H. ( 2013;). Aquimarina longa sp. nov., isolated from seawater, and emended description of Aquimarina muelleri . Int J Syst Evol Microbiol 63 1235–1240 [View Article] [PubMed].
     [Google Scholar]
  56. Yu C., Yu S., Zhang Z., Li Z., Zhang X. H. ( 2014;). Oceanobacillus pacificus sp. nov., isolated from a deep-sea sediment. Int J Syst Evol Microbiol 64 1278–1283 [View Article] [PubMed].
     [Google Scholar]
  57. Zhang D. C., Liu H. C., Xin Y. H., Yu Y., Zhou P. J., Zhou Y. G. ( 2009;). Planomicrobium glaciei sp. nov., a psychrotolerant bacterium isolated from a glacier. Int J Syst Evol Microbiol 59 1387–1390 [View Article] [PubMed].
     [Google Scholar]
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Aureibacillus halotolerans gen. nov., sp. nov., isolated from marine sediment
Int J Syst Evol Microbiol 65, 3950 (2015); https://doi.org/10.1099/ijsem.0.000518
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