1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_11

sp. nov., isolated from a ginseng-cultivated soil Free

Abstract

A dark-pink-coloured bacterial strain, B4Y-8, was isolated from a soil cultivated with ginseng. The 16S rRNA gene sequence of this strain showed highest similarity with BR-18 (96.8 %), BR-3 (96.6 %) and A5 (96.2 %) among the type strains of species of the genus . Strain B4Y-8 was a strictly aerobic, Gram-stain-negative, non-motile, short-rod-shaped bacterium producing a large amount of extracellular polymeric substance. The strain grew at 10–35 °C (optimum, 25 °C), at pH 3.0–11.0 (optimum, pH 7.0) and in the presence of 0–1 % (w/v) NaCl (optimum, 0 %). The DNA G+C content of strain B4Y-8 was 49.0 mol%. It contained menaquinone 7 (MK-7) as the major isoprenoid quinone, and summed feature 3 (Cω6 and/or Cω7) and iso-C as the major fatty acids. On the basis of evidence from the present polyphasic taxonomic study, strain B4Y-8 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is B4Y-8 ( = KACC 18152 = JCM 30759).

  • Published Online:
Funding
This study was supported by the:
  • Research Program for Agricultural Science & Technology Development (Award PJ00898701)
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000519
2015-11-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/11/3933.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000519&mimeType=html&fmt=ahah

References

  1. Ahn J. H., Kim B. C., Kim B. Y., Kim S. J., Song J., Kwon S. W., Weon H. Y. ( 2014;). Paenibacillus cucumis sp. nov. isolated from greenhouse soil. J Microbiol 52 460–464 [View Article] [PubMed].
     [Google Scholar]
  2. Baik K. S., Park S. C., Kim E. M., Lim C. H., Seong C. N. ( 2010;). Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter . Int J Syst Evol Microbiol 60 134–139 [View Article] [PubMed].
     [Google Scholar]
  3. Chen X. Y., Zhao R., Tian Y., Kong B. H., Li X. D., Chen Z. L., Li Y. H. ( 2014;). Mucilaginibacter polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum), and emended description of the genus Mucilaginibacter . Int J Syst Evol Microbiol 64 1395–1400 [View Article] [PubMed].
     [Google Scholar]
  4. Gomori G. ( 1955;). Preparation of buffers for use in enzyme studies. Methods Enzymol 1 138–146 [View Article].
     [Google Scholar]
  5. Gonzalez J. M., Saiz-Jimenez C. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4 770–773 [View Article] [PubMed].
     [Google Scholar]
  6. Hwang Y. M., Baik K. S., Seong C. N. ( 2014;). Mucilaginibacter defluvii sp. nov., isolated from a dye wastewater treatment facility. Int J Syst Evol Microbiol 64 565–571 [View Article] [PubMed].
     [Google Scholar]
  7. Jeon Y., Lee S. S., Chung B. S., Kim J. M., Bae J. W., Park S. K., Jeon C. O. ( 2009;). Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy. Int J Syst Evol Microbiol 59 1451–1454 [View Article] [PubMed].
     [Google Scholar]
  8. Jiang F., Dai J., Wang Y., Xue X., Xu M., Guo Y., Li W., Fang C., Peng F. ( 2012;). Mucilaginibacter soli sp. nov., isolated from Arctic tundra soil. Int J Syst Evol Microbiol 62 1630–1635 [View Article] [PubMed].
     [Google Scholar]
  9. Joung Y., Kim H., Kang H., Lee B. I., Ahn T. S., Joh K. ( 2014;). Mucilaginibacter soyangensis sp. nov., isolated from a lake. Int J Syst Evol Microbiol 64 413–419 [View Article] [PubMed].
     [Google Scholar]
  10. Kämpfer P., Busse H.-J., McInroy J. A., Glaeser S. P. ( 2014;). Mucilaginibacter auburnensis sp. nov., isolated from a plant stem. Int J Syst Evol Microbiol 64 1736–1742 [View Article] [PubMed].
     [Google Scholar]
  11. Kang S. J., Jung Y. T., Oh K. H., Oh T. K., Yoon J. H. ( 2011;). Mucilaginibacter boryungensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 61 1549–1553 [View Article] [PubMed].
     [Google Scholar]
  12. Khan H., Chung E. J., Kang D. Y., Jeon C. O., Chung Y. R. ( 2013;). Mucilaginibacter jinjuensis sp. nov., with xylan-degrading activity. Int J Syst Evol Microbiol 63 1267–1272 [View Article] [PubMed].
     [Google Scholar]
  13. Kim B. C., Lee K. H., Kim M. N., Lee J., Shin K. S. ( 2010;). Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum . FEMS Microbiol Lett 309 130–135 [PubMed].
     [Google Scholar]
  14. Kim B. C., Poo H., Lee K. H., Kim M. N., Kwon O. Y., Shin K. S. ( 2012a;). Mucilaginibacter angelicae sp. nov., isolated from the rhizosphere of Angelica polymorpha Maxim. Int J Syst Evol Microbiol 62 55–60 [View Article] [PubMed].
     [Google Scholar]
  15. Kim J. H., Kang S. J., Jung Y. T., Oh T. K., Yoon J. H. ( 2012b;). Mucilaginibacter lutimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 62 515–519 [View Article] [PubMed].
     [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012c;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  17. Luo X., Zhang L., Dai J., Liu M., Zhang K., An H., Fang C. ( 2009;). Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. Int J Syst Evol Microbiol 59 1447–1450 [View Article] [PubMed].
     [Google Scholar]
  18. Madhaiyan M., Poonguzhali S., Lee J. S., Senthilkumar M., Lee K. C., Sundaram S. ( 2010;). Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. Int J Syst Evol Microbiol 60 2451–2457 [View Article] [PubMed].
     [Google Scholar]
  19. Nakagawa Y., Yamasato K. ( 1993;). Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139 1155–1161 [View Article] [PubMed].
     [Google Scholar]
  20. Pankratov T. A., Tindall B. J., Liesack W., Dedysh S. N. ( 2007;). Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. Int J Syst Evol Microbiol 57 2349–2354 [View Article] [PubMed].
     [Google Scholar]
  21. Pruesse E., Peplies J., Glöckner F. O. ( 2012;). SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28 1823–1829 [View Article] [PubMed].
     [Google Scholar]
  22. Reichenbach H. ( 1992;). The order Cytophagales . . In The Prokaryotes, pp. 3631–3675. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer; [View Article].
     [Google Scholar]
  23. Smibert R. M., Krieg N. R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed].
     [Google Scholar]
  25. Tindall B. J. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13 128–130 [View Article].
     [Google Scholar]
  26. Tindall B. J. ( 1990b;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66 199–202 [View Article].
     [Google Scholar]
  27. Urai M., Aizawa T., Nakagawa Y., Nakajima M., Sunairi M. ( 2008;). Mucilaginibacter kameinonensis sp., nov., isolated from garden soil. Int J Syst Evol Microbiol 58 2046–2050 [View Article] [PubMed].
     [Google Scholar]
  28. Yoon J. H., Kang S. J., Park S., Oh T. K. ( 2012;). Mucilaginibacter litoreus sp. nov., isolated from marine sand. Int J Syst Evol Microbiol 62 2822–2827 [View Article] [PubMed].
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000519
Loading
Mucilaginibacter ginsengisoli sp. nov., isolated from a ginseng-cultivated soil
Int J Syst Evol Microbiol 65, 3933 (2015); https://doi.org/10.1099/ijsem.0.000519
/content/journal/ijsem/10.1099/ijsem.0.000519
/content/journal/ijsem/10.1099/ijsem.0.000519
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error