1887

Abstract

Three Gram-stain-positive, irregular-rod-shaped, non-motile, non-spore-forming bacteria were isolated from nematodes collected from Santa Antao, Cabo Verde (CBX151, CBX152) and Kakegawa, Japan (CBX130). Based on 16S rRNA gene sequence similarity, strains CBX130, CBX151 and CBX152 were shown to belong to the genus . This affiliation was supported by chemotaxonomic data (2,4-diaminobutyric acid in the cell wall; major respiratory quinones MK-10 and MK-11; major polar lipids phosphatidylglycerol and diphosphatidylglycerol; major fatty acids anteiso-C, anteiso-C and iso-C). Strains CBX130 and CBX152 were found to share salient characteristics. Based on morphological, physiological, chemotaxonomic and biochemical analysis, strain CBX152 represents a novel species of the genus , for which the name sp. nov. (type strain CBX152 = DSM 27160 = CIP 110721) is proposed. Two subspecies of sp. nov. are proposed: sp. nov. subsp. subsp. nov. (type strain CBX152 = DSM 27160 = CIP 110721) and sp. nov. subsp. subsp. nov. (type strain CBX130 = DSM 27158 = CIP 110719). The third novel strain, CBX151, showed genetic similarities with NAL101 indicating that these strains belong to the same species. Based on morphological, physiological, chemotaxonomic and biochemical differences it is proposed to split the species into two novel subspecies, subsp. subsp. nov. (type strain NAL101 = KACC 14220 = JCM 16465) and subsp. subsp. nov. (type strain CBX151 = DSM 27159 = CIP 110720), and to emend the description of .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000523
2015-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/11/3977.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000523&mimeType=html&fmt=ahah

References

  1. Behrendt U., Ulrich A., Schumann P. ( 2008;). Leucobacter tardus sp. nov., isolated from the phyllosphere of Solanum tuberosum L. Int J Syst Evol Microbiol 58 25742578 [View Article] [PubMed].
    [Google Scholar]
  2. Brady C., Cleenwerck I., Venter S., Vancanneyt M., Swings J., Coutinho T. ( 2008;). Phylogeny and identification of Pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (MLSA). Syst Appl Microbiol 31 447460 [View Article] [PubMed].
    [Google Scholar]
  3. Brady C. L., Cleenwerck I., Denman S., Venter S. N., Rodríguez-Palenzuela P., Coutinho T. A., De Vos P. ( 2012;). Proposal to reclassify Brenneria quercina (Hildebrand and Schroth 1967) Hauben et al. 1999 into a new genus, Lonsdalea gen. nov., as Lonsdalea quercina comb. nov., descriptions of Lonsdalea quercina subsp. quercina comb. nov., Lonsdalea quercina subsp. iberica subsp. nov. and Lonsdalea quercina subsp. britannica subsp. nov., emendation of the description of the genus Brenneria, reclassification of Dickeya dieffenbachiae as Dickeya dadantii subsp. dieffenbachiae comb. nov., and emendation of the description of Dickeya dadantii . Int J Syst Evol Microbiol 62 15921602 [View Article] [PubMed].
    [Google Scholar]
  4. Dai J., Liu Y., Lei Y., Gao Y., Han F., Xiao Y., Peng H. ( 2011;). A new subspecies of Anoxybacillus flavithermus ssp. yunnanensis ssp. nov. with very high ethanol tolerance. FEMS Microbiol Lett 320 7278 [View Article] [PubMed].
    [Google Scholar]
  5. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  6. Fitch W. M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  7. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M. ( 2007;). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57 8191 [View Article] [PubMed].
    [Google Scholar]
  8. Halpern M., Shakéd T., Pukall R., Schumann P. ( 2009;). Leucobacter chironomi sp. nov., a chromate-resistant bacterium isolated from a chironomid egg mass. Int J Syst Evol Microbiol 59 665670 [View Article] [PubMed].
    [Google Scholar]
  9. Hodgkin J., Félix M.-A., Clark L. C., Stroud D., Gravato-Nobre M. J. ( 2013;). Two Leucobacter strains exert complementary virulence on Caenorhabditis including death by worm-star formation. Curr Biol 23 21572161 [View Article] [PubMed].
    [Google Scholar]
  10. Kim H.-J., Lee S.-S. ( 2011;). Leucobacter kyeonggiensis sp. nov., a new species isolated from dye waste water. J Microbiol 49 10441049 [View Article] [PubMed].
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  12. Konstantinidis K. ( 2007;). Average nucleotide identity calculator. . http://enve-omics.ce.gatech.edu/ani.
  13. Madigan M. T., Martinko J. M., Dunlap P. V., Clark D. P. ( 2009). Brock Biology of Microorganisms , 12th edn.., San Francisco, CA: Benjamin Cummings;.
    [Google Scholar]
  14. Meier-Kolthoff J. P., Auch A. F., Klenk H.-P., Göker M. ( 2013;). Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14 60 [View Article] [PubMed].
    [Google Scholar]
  15. Morais P. V., Francisco R., Branco R., Chung A. P., da Costa M. S. ( 2004;). Leucobacter chromiireducens sp. nov, and Leucobacter aridicollis sp. nov., two new species isolated from a chromium contaminated environment. Syst Appl Microbiol 27 646652 [View Article] [PubMed].
    [Google Scholar]
  16. Muir R. E., Tan M.-W. ( 2007;). Leucobacter chromiireducens subsp. solipictus subsp. nov., a pigmented bacterium isolated from the nematode Caenorhabditis elegans, and emended description of L. chromiireducens . Int J Syst Evol Microbiol 57 27702776 [View Article] [PubMed].
    [Google Scholar]
  17. Percudani R. ( 2013;). A microbial metagenome (Leucobacter sp.) in Caenorhabditis whole genome sequences. Bioinform Biol Insights 7 5572 [View Article] [PubMed].
    [Google Scholar]
  18. Richter M., Rosselló-Móra R. ( 2009;). Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 106 1912619131 [View Article] [PubMed].
    [Google Scholar]
  19. Rutherford K., Parkhill J., Crook J., Horsnell T., Rice P., Rajandream M. A., Barrell B. ( 2000;). Artemis: sequence visualization and annotation. Bioinformatics 16 944945 [View Article] [PubMed].
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  21. Schumann P., Kämpfer P., Busse H.-J., Evtushenko L. I. Subcommittee on the Taxonomy of the Suborder Micrococcineae of the International Committee on Systematics of Prokaryotes ( 2009;). Proposed minimal standards for describing new genera and species of the suborder Micrococcineae . Int J Syst Evol Microbiol 59 18231849 [View Article] [PubMed].
    [Google Scholar]
  22. Shin N.-R., Kim M.-S., Jung M.-J., Roh S. W., Nam Y.-D., Park E.-J., Bae J.-W. ( 2011;). Leucobacter celer sp. nov., isolated from Korean fermented seafood. Int J Syst Evol Microbiol 61 23532357 [View Article] [PubMed].
    [Google Scholar]
  23. Somvanshi V. S., Lang E., Schumann P., Pukall R., Kroppenstedt R. M., Ganguly S., Stackebrandt E. ( 2007;). Leucobacter iarius sp. nov., in the family Microbacteriaceae . Int J Syst Evol Microbiol 57 682686 [View Article] [PubMed].
    [Google Scholar]
  24. Sturm G., Jacobs J., Spröer C., Schumann P., Gescher J. ( 2011;). Leucobacter chromiiresistens sp. nov., a chromate-resistant strain. Int J Syst Evol Microbiol 61 956960 [View Article] [PubMed].
    [Google Scholar]
  25. Sturm G., Buchta K., Kurz T., Rensing S. A., Gescher J. ( 2012;). Draft genome sequence of Leucobacter chromiiresistens, an extremely chromium-tolerant strain. J Bacteriol 194 540541 [View Article] [PubMed].
    [Google Scholar]
  26. Takeuchi M., Weiss N., Schumann P., Yokota A. ( 1996;). Leucobacter komagatae gen. nov., sp. nov., a new aerobic gram-positive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46 967971 [View Article] [PubMed].
    [Google Scholar]
  27. Tamura K., Nei M. ( 1993;). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10 512526 [PubMed].
    [Google Scholar]
  28. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed].
    [Google Scholar]
  29. Teeling H., Meyerdierks A., Bauer M., Amann R., Glöckner F. O. ( 2004;). Application of tetranucleotide frequencies for the assignment of genomic fragments. Environ Microbiol 6 938947 [View Article] [PubMed].
    [Google Scholar]
  30. Trutko S. M., Dorofeeva L. V., Evtushenko L. I., Ostrovskiî D. N., Hintz M., Wiesner J., Jomaa H., Baskunov B. P., Akimenko V. K. ( 2005;). [Isoprenoid pigments in representatives of the family Microbacteriaceae]. Mikrobiologiia 74 335341 (in Russian) [PubMed].
    [Google Scholar]
  31. Ue H. ( 2011;). Leucobacter exalbidus sp. nov., an actinobacterium isolated from a mixed culture from compost. J Gen Appl Microbiol 57 2733 [View Article] [PubMed].
    [Google Scholar]
  32. Yun J.-H., Roh S. W., Kim M.-S., Jung M.-J., Park E.-J., Shin K.-S., Nam Y.-D., Bae J.-W. ( 2011;). Leucobacter salsicius sp. nov., from a salt-fermented food. Int J Syst Evol Microbiol 61 502506 [View Article] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000523
Loading
/content/journal/ijsem/10.1099/ijsem.0.000523
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error