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Volume 65, Issue Pt_11

gen. nov., sp. nov., isolated from rice paddy soil Free

Abstract

A mesophilic, aerobic, Gram-stain-positive, filamentous bacterial strain, designated ZYf1a3, was isolated from rice paddy soil in Japan. This strain grew on a solid medium with formation of substrate mycelium; endospores were produced singly along the mycelium. Formation of aerial mycelium was not observed on any of the media tested. This strain produced a characteristic saffron yellow soluble pigment. Cloned 16S rRNA gene sequences of strain ZYf1a3 yielded three different copies (similarity between the three sequences: 99.8–99.9 %). One of these sequences had one base deletion. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain ZYf1a3 belongs to an independent phylogenetic lineage of the family . The cell wall of strain ZYf1a3 contained -diaminopimelic acid, alanine and glutamic acid, but no characteristic sugars. It contained menaquinone 7 as the sole menaquinone. The major cellular fatty acids were iso-C and anteiso-C.The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl--methylethanolamine and unidentified aminophospholipids. The DNA G+C content was 42.5 mol%. From phylogenetic analysis based on 16S rRNA gene sequences and phenotypic characteristics, this strain is considered to represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is ZYf1a3 ( = JCM 30426 = CCUG 66446 = DSM 46876).

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2015-11-01
2024-04-18
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Croceifilum oryzae gen. nov., sp. nov., isolated from rice paddy soil
Int J Syst Evol Microbiol 65, 4061 (2015); https://doi.org/10.1099/ijsem.0.000537
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