1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_11

sp. nov., isolated from seawater of the South Pacific Gyre Free

Abstract

A Gram-stain-negative, strictly aerobic, non-flagellated, non-gliding, oxidase- and catalase-positive, rod-shaped and orange-pigmented bacterium with appendages, designated strain SW027, was isolated from a surface seawater sample collected from the South Pacific Gyre (26° 29′ S 137° 56′ W) during the Integrated Ocean Drilling Program (IODP) Expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SW027 shared the highest sequence similarity with members of the genus (94.3–92.7 %), exhibiting 94.3 % sequence similarity to SW-62. Optimal growth occurred in the presence of 3 % (w/v) NaCl, at pH 7.0 and at 37 °C. The DNA G+C content of strain SW027 was 42.7 mol%. The major fatty acids were iso-C, iso-C G and iso-C 3-OH. The major respiratory quinone was menaquinone-6. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. Enzymic activity profiles, cell morphology and DNA G+C content differentiated the novel bacterium from the most closely related members of the genus . On the basis of the polyphasic analyses, strain SW027 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SW027 ( = JCM 17861 = LMG 26637).

  • Published Online:
Funding
This study was supported by the:
  • the National Natural Science Foundation of China (Award no. 41276141)
  • the China Ocean Mineral Resources Research & Development Association (Award no. DY125-15-R-03)
  • the National High Technology Research & Development Program of China (Award no. 2012AA091605)
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000542
2015-11-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/11/4087.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000542&mimeType=html&fmt=ahah

References

  1. Arun A.B., Chen W.-M., Lai W.-A., Chao J.-H., Rekha P.D., Shen F.-T., Singh S., Young C.-C. ( 2009;). Muricauda lutaonensis sp. nov., a moderate thermophile isolated from a coastal hot spring. Int J Syst Evol Microbiol 59 2738–2742 [View Article] [PubMed].
     [Google Scholar]
  2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. ), ( 1995). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn., New York: Wiley;.
     [Google Scholar]
  3. Bernardet J.F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 1049–1070 [View Article] [PubMed].
     [Google Scholar]
  4. Beveridge T.J., Lawrence J.R., Murray R.G.E. ( 2007;). Sampling and staining for light microscopy. . In Methods for General and Molecular Microbiology, pp. 19–33. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  5. Bruns A., Rohde M., Berthe-Corti L. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51 1997–2006 [View Article] [PubMed].
     [Google Scholar]
  6. Collins M.D., Shah H.N. ( 1984;). Fatty acid, menaquinone and polar lipid composition of Rothia dentosacariosa . Arch Microbiol 137 247–249 [View Article].
     [Google Scholar]
  7. Hameed A., Arun A.B., Ho H.-P., Chang C.-M.J., Rekha P.D., Lee M.-R., Singh S., Young C.-C. ( 2011;). Supercritical carbon dioxide micronization of zeaxanthin from moderately thermophilic bacteria Muricauda lutaonensis CC-HSB-11T . J Agric Food Chem 59 4119–4124 [View Article] [PubMed].
     [Google Scholar]
  8. Hwang C.Y., Kim M.H., Bae G.D., Zhang G.I., Kim Y.H., Cho B.C. ( 2009;). Muricauda olearia sp. nov., isolated from crude-oil-contaminated seawater, and emended description of the genus Muricauda . Int J Syst Evol Microbiol 59 1856–1861 [View Article] [PubMed].
     [Google Scholar]
  9. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  10. Kim J.M., Jin H.M., Jeon C.O. ( 2013;). Muricauda taeanensis sp. nov., isolated from a marine tidal flat. Int J Syst Evol Microbiol 63 2672–2677 [View Article] [PubMed].
     [Google Scholar]
  11. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111–120 [View Article] [PubMed].
     [Google Scholar]
  12. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161–207. [CrossRef]
    [Google Scholar]
  13. Lee S.-Y., Park S., Oh T.-K., Yoon J.-H. ( 2012;). Muricauda beolgyonensis sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 62 1134–1139 [View Article] [PubMed].
     [Google Scholar]
  14. Mesbah M., Whitman W.B. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr A 479 297–306 [View Article] [PubMed].
     [Google Scholar]
  15. Minnikin D., O'Donnell A., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241 [View Article].
     [Google Scholar]
  16. Montero-Calasanz M.delC., Göker M., Rohde M., Spröer C., Schumann P., Busse H.J., Schmid M., Tindall B.J., Klenk H.P., Camacho M. ( 2013;). Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium . Int J Syst Evol Microbiol 63 4386–4395 [View Article] [PubMed].
     [Google Scholar]
  17. Moore E.R.B., Arnscheidt A., Krüger A., Strömpl C., Mau M. ( 1999;). Simplified protocols for the preparation of genomic DNA from bacterial cultures. . In Molecular Microbial Ecology Manual 1.6.1, pp. 1–15. Edited by Akkermans A. D. L., van Elsas J. D., de Bruijn F. J. Dordrecht: Kluwer;.
     [Google Scholar]
  18. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  19. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. 44 846–849.
     [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed].
     [Google Scholar]
  21. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882 [View Article] [PubMed].
     [Google Scholar]
  22. Tindall B.J., Sikorski J., Smibert R.M., Krieg N.R. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. , 3rd edn.., Washington, DC: American Society for Microbiology;. [View Article].
     [Google Scholar]
  23. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 697–703 [PubMed].
     [Google Scholar]
  24. Wu Y.-H., Yu P.-S., Zhou Y.-D., Xu L., Wang C.-S., Wu M., Oren A., Xu X.-W. ( 2013;). Muricauda antarctica sp. nov., a marine member of the Flavobacteriaceae isolated from Antarctic seawater. Int J Syst Evol Microbiol 63 3451–3456 [View Article] [PubMed].
     [Google Scholar]
  25. Yang C., Li Y., Guo Q., Lai Q., Wei J., Zheng T., Tian Y. ( 2013;). Muricauda zhangzhouensis sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 63 2320–2325. [CrossRef]
    [Google Scholar]
  26. Yoon J.H., Lee K.C., Kho Y.H., Kang K.H., Kim C.J., Park Y.H. ( 2002;). Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52 123–130 [PubMed]. [CrossRef]
    [Google Scholar]
  27. Yoon J.-H., Lee M.-H., Oh T.-K., Park Y.-H. ( 2005;). Muricauda flavescens sp. nov. and Muricauda aquimarina sp. nov., isolated from a salt lake near Hwajinpo Beach of the East Sea in Korea, and emended description of the genus Muricauda . Int J Syst Evol Microbiol 55 1015–1019 [View Article] [PubMed].
     [Google Scholar]
  28. Yoon J.-H., Kang S.-J., Jung Y.-T., Oh T.-K. ( 2008;). Muricauda lutimaris sp. nov., isolated from a tidal flat of the Yellow Sea. Int J Syst Evol Microbiol 58 1603–1607 [View Article] [PubMed].
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000542
Loading
Muricauda pacifica sp. nov., isolated from seawater of the South Pacific Gyre
Int J Syst Evol Microbiol 65, 4087 (2015); https://doi.org/10.1099/ijsem.0.000542
/content/journal/ijsem/10.1099/ijsem.0.000542
/content/journal/ijsem/10.1099/ijsem.0.000542
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error