RT Journal Article SR Electronic(1) A1 Vaz-Moreira, Ivone A1 Narciso-da-Rocha, Carlos A1 De Brandt, Evie A1 Vandamme, Peter A1 Silva Ferreira, A. C. A1 Lobo-da-Cunha, Alexandre A1 Nunes, Olga C. A1 Manaia, Célia M.YR 2015 T1 Hydromonas duriensis gen. nov., sp. nov., isolated from freshwater JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_11 SP 4134 OP 4139 DO https://doi.org/10.1099/ijsem.0.000546 PB Microbiology Society, SN 1466-5034, AB An aerobic, Gram-stain-negative rod, designated strain A2P5T, was isolated from the Douro river, in Porto, Portugal. Cells were catalase- and oxidase-positive. Growth occurred at 15–30 °C, at pH 6–8 and in the presence of 1 % (w/v) NaCl. The major respiratory quinone was Q8, the genomic DNA had a G+C content of 47 ± 1 mol%, and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol were amongst the major polar lipids. On the basis of 16S rRNA gene sequence analysis, strain A2P5T was observed to be a member of the family Burkholderiaceae, but could not be identified as a member of any validly named genus. The low levels of 16S rRNA gene sequence similarity to other recognized taxa ( < 91 %), together with the comparative analysis of phenotypic and chemotaxonomic characteristics, supported the proposal of a novel species of a new genus within the family Burkholderiaceae. The name Hydromonas duriensis gen. nov., sp. nov. is proposed. The type strain of Hydromonas duriensis is A2P5T ( = LMG 28428T = CCUG 66137T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000546