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f gen. nov., sp. nov., isolated from deep seawater

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  • Authors: Guizhen Li1​ ,2​ ,3 ,  Qiliang Lai2​ ,3 ,  Yaping Du2​ ,  Xiupian Liu2​ ,  Fengqin Sun2​ , Zongze Shao1​ ,2​
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    • 1​ 1​ School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China 2​ 2​ State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China
  • Zongze Shao [email protected]
  • First Published Online: 01 November 2015, International Journal of Systematic and Evolutionary Microbiology 65: 4175-4179, doi: 10.1099/ijsem.0.000557
  • Subject: NEW TAXA - Proteobacteria
  • Cover date:
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  • A taxonomic study was carried out on strain 22II1-22F33, which was isolated from deep seawater of the Atlantic Ocean. The bacterium was Gram-stain-negative, oxidase-positive and weakly catalase-positive, oval in shape without flagellum. Growth was observed at salinities of 0–12 % and at temperatures of 4–41 °C. The isolate was capable of hydrolysing aesculin and Tween 80 and reduction of nitrate to nitrite, but unable to hydrolyse gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II1-22F33 belongs to the family , with highest sequence similarity to AZO-C (96.5 %). The principal fatty acids (>10 %) were summed feature 8 (Cω7/ω6c) (73.8 %). The G+C content of the genomic DNA was 66.2 mol%. The respiratory quinone was Q-10 (100 %). Phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), two unidentified aminolipids (ALs), six unidentified phospholipids (PLs) and one unidentified lipid (L) were present. The combined genotypic and phenotypic data show that strain 22II1-22F33 represents a novel species within a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is 22II1-22F33 ( = LMG 27151 = MCCC 1A09326).

  • These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of 22II1-22F33 is KT223370.

  • Four supplementary figures are available with the online Supplementary Material.

© 2015 IUMS | Published by the Microbiology Society

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2015-11-01
2019-02-19
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