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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_11

sp. nov., isolated from noni ( L.) branch Free

Abstract

A Gram-stain-positive bacterial strain, designated as NR2, isolated from noni ( L.) branch was investigated using a polyphasic taxonomic approach. The cells were small coccoid to ovoid, non-spore-forming and motile. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was a representative of a member of the genus , to which the most closely related neighbours were M-6-3 (97.90 % similarity), DSM 4810 (97.50 %), LMG 20345 (97.41 %), phenol-A (97.36 %), DSM 9573 (97.36 %) and LMG 19848 (97.32 %). The polar lipid profile of strain NR2 consisted of diphosphatidylglycerol, phosphatidylglycerol, unknown phospholipids and unknown glycolipids. The predominant respiratory quinone was MK-8, with MK-9 and MK-7 as minor components. The major fatty acids were anteiso-C and iso-C. Strain NR2 was clearly distinguishable from the type strains of related species on the basis of phylogenetic analysis, DNA–DNA hybridization, fatty acid composition data analysis and a range of physiological and comparison of biochemical characteristics. It is evident from the genotypic and phenotypic data that strain NR2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NR2 ( = DSM 29535 = CICC 10874).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31300008)
  • Fund of National Infrastructure of Microbial Resources (Award NIMR2015-4)
  • Beijing Nova Program (Award Z141105001814095)
  • Scientific and Technological Development Project of China National Research Institute of Food and Fermentation Industries (Award 2012KJFZ-BS-01)
© 2015 IUMS
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2015-11-01
2024-04-24
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Brachybacterium hainanense sp. nov., isolated from noni (Morinda citrifolia L.) branch
Int J Syst Evol Microbiol 65, 4196 (2015); https://doi.org/10.1099/ijsem.0.000559
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