RT Journal Article SR Electronic(1) A1 Liu, Yang A1 Zhai, Lei A1 Yao, Su A1 Cao, Yanhua A1 Cao, Yu A1 Zhang, Xin A1 Su, Jiaojiao A1 Ge, Yuanyuan A1 Zhao, Ran A1 Cheng, ChiYR 2015 T1 Brachybacterium hainanense sp. nov., isolated from noni (Morinda citrifolia L.) branch JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_11 SP 4196 OP 4201 DO https://doi.org/10.1099/ijsem.0.000559 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive bacterial strain, designated as NR2T, isolated from noni (Morinda citrifolia L.) branch was investigated using a polyphasic taxonomic approach. The cells were small coccoid to ovoid, non-spore-forming and motile. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was a representative of a member of the genus Brachybacterium, to which the most closely related neighbours were Brachybacterium squillarum M-6-3T (97.90 % similarity), Brachybacterium faecium DSM 4810T (97.50 %), Brachybacterium sacelli LMG 20345T (97.41 %), Brachybacterium phenoliresistens phenol-AT (97.36 %), Brachybacterium nesterenkovii DSM 9573T (97.36 %) and Brachybacterium rhamnosum LMG 19848T (97.32 %). The polar lipid profile of strain NR2T consisted of diphosphatidylglycerol, phosphatidylglycerol, unknown phospholipids and unknown glycolipids. The predominant respiratory quinone was MK-8, with MK-9 and MK-7 as minor components. The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. Strain NR2T was clearly distinguishable from the type strains of related species on the basis of phylogenetic analysis, DNA–DNA hybridization, fatty acid composition data analysis and a range of physiological and comparison of biochemical characteristics. It is evident from the genotypic and phenotypic data that strain NR2T represents a novel species of the genus Brachybacterium, for which the name Brachybacterium hainanense sp. nov. is proposed. The type strain is NR2T ( = DSM 29535T = CICC 10874T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000559