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f gen. nov., sp. nov., a halophilic actinomycete

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  • A novel halophilic actinobacterium, designated strain TRM 49201, was isolated from a hypersaline soil in Xinjiang Province, north-west China. The strain was aerobic, Gram-stain-positive and halophilic. The aerial mycelium was chaotic with irregular branches, and spherical sporangia containing several spherical spores developed at mycelial aggregations. The strain had an optimum NaCl concentration for growth of 8–13 % (w/v). The whole-cell sugar pattern of strain TRM 49201 consisted of xylose and ribose. The predominant menaquinones were MK-9(H), MK-9(H) and MK-10(H). The polar lipids were diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phosphatidylglycerol, phosphatidylcholine and four unknown phospholipids. The major fatty acids were anteiso-C, iso-C and anteiso-C. The G+C content of the genomic DNA was 70 mol%. Phylogenetic analysis showed that strain TRM 49201 can be distinguished from representatives of , and , the three existing genera in the family , based on low 16S rRNA gene sequence similarities ( < 94.42 %). Strain TRM 49201 is thus considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is TRM 49201 ( = NRRL B-24926 = CCTCC AA 2013002 = KACC 17683).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain TRM 49201 gene for 16S rRNA is AB857719.

  • Six supplementary figures are available with the online Supplementary Material.

© 2015 IUMS | Published by the Microbiology Society

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2015-11-01
2019-02-20
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