1887

Abstract

Three strains of anaerobic Gram-stain-negative, short to longer rod-shaped bacteria isolated from the caecum of chicken in Indonesia were studied using a polyphasic taxonomic approach. These strains belonged to the genus , based on sequence analysis of 16S rRNA and () genes, with similarities of 93.2–94.1 and 89.8–90.8 %, respectively, to the closest recognized species, JCM 17929. Sugar fermentation and enzyme characteristics, cellular fatty acid profiles, menaquinone profiles and metabolic end products were also investigated. Furthermore, DNA–DNA hybridization studies confirmed that the three novel strains are different from the closest related species. The strains were also found to be distinct from each other on the basis of ribotype profiles. The DNA G+C contents of the three strains were 41.1–41.8 mol%. Based on phenotypic and phylogenetic characteristics, a novel species, sp. nov., is proposed (type strain C13EG111 = LIPI12-4-Ck773 = JSAT12-4-Ck773 = InaCC B455 = NBRC 110959).

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2015-12-01
2024-03-28
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