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Abstract

A polyphasic taxonomic study was performed on an unidentified -like, Gram-stain-positive bacterium, strain 2710, isolated from a harbour seal. Comparative 16S rRNA gene sequence analysis showed that this bacterial strain belonged to the genus and was related most closely to the type strains of (98.4 % similarity) and (97.5 %). 16S rRNA gene sequence similarities to the type strains of other species were between 95.3 and 96.9 %. DNA–DNA hybridization values between strain 2710 and DSM 10002 and LMG 27073 were 4.7 % (reciprocal 56 %) and 23 % (reciprocal 7.7 %), respectively. The presence of the major menaquinone MK-9(H4) and a polar lipid profile with the major compounds diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside supported the affiliation of strain 2710 to the genus . The major fatty acids were C16:0, C18:1ω9, C18:0 and C18:2ω6,9/anteiso-C18:0. The peptidoglycan structure was of cross-linkage type A5α (-Lys–-Lys–-Glu). Physiological and biochemical tests clearly distinguished the isolate from other members of the genus . Based on these tests, it is proposed that this unknown bacterium should be classified as a novel species of the genus , with the name sp. nov. The type strain is 2710 ( = DSM 28752 = LMG 28298).

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2015-12-01
2024-03-28
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H. J. ( 1996;). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47 3952 [View Article].
    [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. ( 1978;). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75 48014805 [View Article] [PubMed] .
    [Google Scholar]
  3. Collins M. D., Jones D., Schofield G. M. ( 1982;). Reclassification of ‘Corynebacterium haemolyticum’ (MacLean, Liebow & Rosenberg) in the genus Arcanobacterium gen.nov. as Arcanobacterium haemolyticum nom.rev., comb.nov. J Gen Microbiol 128 12791281 [PubMed].
    [Google Scholar]
  4. Felsenstein J. ( 1985;). Confidence limits of phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  5. Felsenstein J. ( 2005). phylip (phylogeny inference package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA .
  6. Hijazin M., Prenger-Berninghoff E., Sammra O., Alber J., Lämmler C., Kämpfer P., Glaeser S. P., Busse H. J., Hassan A. A., other authors. ( 2012;). Arcanobacterium canis sp. nov., isolated from otitis externa of a dog, and emended description of the genus Arcanobacterium Collins et al. 1983 emend. Yassin et al. 2011. Int J Syst Evol Microbiol 62 22012205 [View Article] [PubMed] .
    [Google Scholar]
  7. Hijazin M., Sammra O., Ülbegi-Mohyla H., Nagib S., Alber J., Lämmler C., Kämpfer P., Glaeser S. P., Busse H. J., other authors. ( 2013;). Arcanobacterium phocisimile sp. nov., isolated from harbour seals. Int J Syst Evol Microbiol 63 20192024 [View Article] [PubMed] .
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. ( 1969;). Evolution of the protein molecules. . In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. N. [View Article] Academic Press;.
    [Google Scholar]
  9. Kämpfer P., Kroppenstedt R. M. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42 9891005 [View Article].
    [Google Scholar]
  10. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., other authors. ( 2004;). arb: a software environment for sequence data. Nucleic Acids Res 32 13631371 [View Article] [PubMed] .
    [Google Scholar]
  11. Pitcher D. D., Saunders N. A., Owen R. J. ( 1989;). Rapid extraction of bacterial genomic DNA with guanidinium thiocyanate. Lett Appl Microbiol 8 151156 [View Article].
    [Google Scholar]
  12. Pruesse E., Peplies J., Glöckner F. O. ( 2012;). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28 18231829 [View Article] [PubMed] .
    [Google Scholar]
  13. Sammra O., Balbutskaya A., Nagib S., Alber J., Lämmler C., Abdulmawjood A., Timke M., Kostrzewa M., Prenger-Berninghoff E. ( 2014;). Properties of an Arcanobacterium haemolyticum strain isolated from a donkey. Berl Munch Tierarztl Wochenschr 127 5660 [PubMed].
    [Google Scholar]
  14. Schumann P. ( 2011;). Peptidoglycan structure. Methods Microbiol 38 101129 [View Article].
    [Google Scholar]
  15. Stamatakis A. ( 2006;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22 26882690 [View Article] [PubMed] .
    [Google Scholar]
  16. Stolz A., Busse H. J., Kämpfer P. ( 2007;). Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57 572576 [View Article] [PubMed] .
    [Google Scholar]
  17. Tindall B. J. ( 1990a;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66 199202 [View Article].
    [Google Scholar]
  18. Tindall B. J. ( 1990b;). A comparative study of the lipid composition of Halobacterium sascharovorum from various sources. Syst Appl Microbiol 13 128130 [View Article].
    [Google Scholar]
  19. Ülbegi-Mohyla H., Hassan A. A., Kanbar T., Alber J., Lämmler C., Prenger-Berninghoff E., Weiss R., Siebert U., Zschöck M. ( 2009;). Synergistic and antagonistic hemolytic activities of bacteria of genus Arcanobacterium and CAMP-like hemolysis of Arcanobacterium phocae and Arcanobacterium haemolyticum with Psychrobacter phenylpyruvicus . Res Vet Sci 87 186188 [View Article] [PubMed] .
    [Google Scholar]
  20. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R. ( 2008;). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 31 241250 [View Article] [PubMed] .
    [Google Scholar]
  21. Yassin A. F., Hupfer H., Siering C., Schumann P. ( 2011;). Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al. 1982 emend. Lehnen et al. 2006: proposal for Trueperella gen. nov. and emended description of the genus Arcanobacterium . Int J Syst Evol Microbiol 61 12651274 [View Article] [PubMed] .
    [Google Scholar]
  22. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. ( 1998;). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48 179186 [View Article] [PubMed] .
    [Google Scholar]
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