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Volume 65, Issue Pt_12

gen. nov., sp. nov., isolated from surface water of a saline lake Free

Abstract

A Gram-stain-negative bacterium, strain TS-T30, was isolated from a saline lake (Lake Tuosu) in Qaidam basin, Qinghai province, China, and its taxonomic position was determined by using a polyphasic approach. Cells were non-spore-forming rods, non-motile, 0.8–1.4 μm wide and 1.9–4.0 μm long. Strain TS-T30 was strictly heterotrophic and aerobic. Catalase- and oxidase-positive. Growth was observed in the presence of 0.5–11.0 % (w/v) NaCl (optimum 3.0 %), and at 10–35 °C (optimum 25 °C) and pH 6.5–10.0 (optimum pH 8.5). Strain TS-T30 contained Cω7 as the only predominant fatty acid. The major respiratory quinone was Q-10. The DNA G+C content was 62 mol% ( ). Phylogenetic trees based on 16S rRNA gene sequences showed that strain TS-T30 formed a distinct lineage that was independent of other most closely related genera: (95.2–95.9 % 16S rRNA gene sequence similarities), (95.4 %), (92.8–94.9 %), (93.6–94.9 %), (94.7 %), (94.7 %), (93.9–94.7 %), (92.6–94.5 %) and (94.5 %). The major polar lipids were phosphatidylglycerol, one unidentified phospholipid and an unknown aminolipid; phosphatidylcholine was not detected. These data demonstrated that strain TS-T30 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is TS-T30 ( = CGMCC 1.12477 = NBRC 110969).

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2015-12-01
2024-04-24
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990;). Basic local alignment search tool. J Mol Biol 215 403–410 [View Article] [PubMed].
     [Google Scholar]
  2. Chakravarthy S. K., Sucharitha K., Sasikala Ch., Ramana ChV. ( 2009;). Rhodovulum lacipunicei sp. nov., an obligate sulfide-demanding phototrophic alphaproteobacterium isolated from a purple pond in India. Int J Syst Evol Microbiol 59 1615–1619 [View Article] [PubMed].
     [Google Scholar]
  3. Chen M. H., Sheu S. Y., Chen C. A., Wang J. T., Chen W. M. ( 2011;). Shimia isoporae sp. nov., isolated from the reef-building coral Isopora palifera . Int J Syst Evol Microbiol 61 823–827 [View Article] [PubMed].
     [Google Scholar]
  4. Chen W. M., Cho N. T., Huang W. C., Young C. C., Sheu S. Y. ( 2013;). Description of Gemmobacter fontiphilus sp. nov., isolated from a freshwater spring, reclassification of Catellibacterium nectariphilum as Gemmobacter nectariphilus comb. nov., Catellibacterium changlense as Gemmobacter changlensis comb. nov., Catellibacterium aquatile as Gemmobacter aquaticus nom. nov., Catellibacterium caeni as Gemmobacter caeni comb. nov., Catellibacterium nanjingense as Gemmobacter nanjingensis comb. nov., and emended description of the genus Gemmobacter and of Gemmobacter aquatilis . Int J Syst Evol Microbiol 63 470–478 [View Article] [PubMed].
     [Google Scholar]
  5. Cho J. C., Giovannoni S. J. ( 2004;). Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly-beta-hydroxybutyrate-producing marine bacteria in the order ‘Rhodobacterales’. Int J Syst Evol Microbiol 54 1129–1136 [View Article] [PubMed].
     [Google Scholar]
  6. Choi D. H., Cho B. C. ( 2006;). Shimia marina gen. nov., sp. nov., a novel bacterium of the Roseobacter clade isolated from biofilm in a coastal fish farm. Int J Syst Evol Microbiol 56 1869–1873 [View Article] [PubMed].
     [Google Scholar]
  7. Collins M. D. ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E. London: Academic Press;.
     [Google Scholar]
  8. Dong X. Z., Cai M. Y., (editors). ( 2001). Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);.
     [Google Scholar]
  9. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376 [View Article] [PubMed].
     [Google Scholar]
  10. Fitch W. M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416 [View Article].
     [Google Scholar]
  11. Garrity G. M., Bell J. A., Lilburn T. III ( 2005;). Order III. Rhodobacterales ord. nov. . In Bergey's Manual of Systematic Bacteriology, 2nd edn., vol. 2 (The Proteobacteria), part C, p. 161. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;.
     [Google Scholar]
  12. Garrity G. M., Bell J. A., Lilburn T. ( 2006;). Rhodobacterales ord. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 107. Int J Syst Evol Microbiol 56 1–6 [View Article] [PubMed].
     [Google Scholar]
  13. Gu J., Guo B., Wang Y. N., Yu S. L., Inamori R., Qu R., Ye Y. G., Wu X. L. ( 2007;). Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea. Int J Syst Evol Microbiol 57 157–160 [View Article] [PubMed].
     [Google Scholar]
  14. Hahnke S., Tindall B. J., Schumann P., Simon M., Brinkhoff T. ( 2013;). Pelagimonas varians gen. nov., sp. nov., isolated from the southern North Sea. Int J Syst Evol Microbiol 63 835–843 [View Article] [PubMed].
     [Google Scholar]
  15. Hameed A., Shahina M., Lin S. Y., Lai W. A., Hsu Y. H., Liu Y. C., Huang Y. M., Young C. C. ( 2013;). Shimia biformata sp. nov., isolated from surface seawater, and emended description of the genus Shimia Choi and Cho 2006. Int J Syst Evol Microbiol 63 4533–4539 [View Article] [PubMed].
     [Google Scholar]
  16. Huo Y. Y., Xu X. W., Li X., Liu C., Cui H. L., Wang C. S., Wu M. ( 2011;). Ruegeria marina sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 61 347–350 [View Article] [PubMed].
     [Google Scholar]
  17. Huo Y. Y., Li Z. Y., You H., Wang C. S., Post A. F., Oren A., Xu X. W. ( 2014;). Oceanicola antarcticus sp. nov. and Oceanicola flagellatus sp. nov., moderately halophilic bacteria isolated from seawater. Int J Syst Evol Microbiol 64 2975–2979 [View Article] [PubMed].
     [Google Scholar]
  18. Hwang C. Y., Bae G. D., Yih W., Cho B. C. ( 2009;). Marivita cryptomonadis gen. nov., sp. nov. and Marivita litorea sp. nov., of the family Rhodobacteraceae, isolated from marine habitats. Int J Syst Evol Microbiol 59 1568–1575 [View Article] [PubMed].
     [Google Scholar]
  19. Hyun D. W., Kim M. S., Shin N. R., Kim J. Y., Kim P. S., Whon T. W., Yun J. H., Bae J. W. ( 2013;). Shimia haliotis sp. nov., a bacterium isolated from the gut of an abalone, Haliotis discus hannai . Int J Syst Evol Microbiol 63 4248–4253 [View Article] [PubMed].
     [Google Scholar]
  20. Imhoff J. F., Caumette P. ( 2004;). Recommended standards for the description of new species of anoxygenic phototrophic bacteria. Int J Syst Evol Microbiol 54 1415–1421 [View Article] [PubMed].
     [Google Scholar]
  21. Imhoff J. F., Trüper H. G., Pfennig N. ( 1984;). Rearrangement of the species and genera of the phototrophic “purple nonsulfur bacteria”. Int J Syst Bacteriol 34 340–343 [View Article].
     [Google Scholar]
  22. Iwaki H., Yasukawa N., Fujioka M., Takada K., Hasegawa Y. ( 2013;). Isolation and characterization of a marine cyclohexylacetate-degrading bacterium Lutimaribacter litoralis sp. nov., and reclassification of Oceanicola pacificus as Lutimaribacter pacificus comb. nov. Curr Microbiol 66 588–593 [View Article] [PubMed].
     [Google Scholar]
  23. Kämpfer P., Arun A. B., Rekha P. D., Busse H. J., Young C. C., Glaeser S. P. ( 2013;). Ruegeria intermedia sp. nov., a moderately thermophilic bacterium isolated from a coastal hot spring. Int J Syst Evol Microbiol 63 2538–2544 [View Article] [PubMed].
     [Google Scholar]
  24. Kates M. ( 1986). Techniques of Lipidology , 2nd edn. Amsterdam: Elsevier;.
     [Google Scholar]
  25. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012a;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  26. Kim Y. O., Park S., Nam B. H., Kang S. J., Hur Y. B., Lee S. J., Oh T. K., Yoon J. H. ( 2012b;). Ruegeria halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi . Int J Syst Evol Microbiol 62 925–930 [View Article] [PubMed].
     [Google Scholar]
  27. Kim Y. O., Park S., Nam B. H., Jung Y. T., Kim D. G., Yoon J. H. ( 2014;). Ruegeria meonggei sp. nov., an alphaproteobacterium isolated from ascidian Halocynthia roretzi . Antonie van Leeuwenhoek 105 551–558 [View Article] [PubMed].
     [Google Scholar]
  28. Kimura M. ( 1983). The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [View Article].
     [Google Scholar]
  29. Labrenz M., Lawson P. A., Tindall B. J., Collins M. D., Hirsch P. ( 2005;). Roseisalinus antarcticus gen. nov., sp. nov., a novel aerobic bacteriochlorophyll a-producing α-proteobacterium isolated from hypersaline Ekho Lake, Antarctica. Int J Syst Evol Microbiol 55 41–47 [View Article] [PubMed].
     [Google Scholar]
  30. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A., other authors. ( 2007;). clustal w clustal_x version 2.0. Bioinformatics 23 2947–2948 [View Article] [PubMed].
     [Google Scholar]
  31. Lee J., Whon T. W., Shin N. R., Roh S. W., Kim J., Park S. K., Kim M. S., Shin K. S., Lee J. S., other authors. ( 2012;). Ruegeria conchae sp. nov., isolated from the ark clam Scapharca broughtonii . Int J Syst Evol Microbiol 62 2851–2857 [View Article] [PubMed].
     [Google Scholar]
  32. Lin K. Y., Sheu S. Y., Chang P. S., Cho J. C., Chen W. M. ( 2007;). Oceanicola marinus sp. nov., a marine alphaproteobacterium isolated from seawater collected off Taiwan. Int J Syst Evol Microbiol 57 1625–1629 [View Article] [PubMed].
     [Google Scholar]
  33. LPSN ( 2015). http://www.bacterio.net/-classifgenerafamilies.html#Rhodobacteraceae List of Prokaryotic names with Standing in Nomenclature..
  34. Ludwig W., Mittenhuber G., Friedrich C. G. ( 1993;). Transfer of Thiosphaera pantotropha to Paracoccus denitrificans . Int J Syst Bacteriol 43 363–367 [View Article] [PubMed].
     [Google Scholar]
  35. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109–118 [View Article] [PubMed].
     [Google Scholar]
  36. Muramatsu Y., Uchino Y., Kasai H., Suzuki K., Nakagawa Y. ( 2007;). Ruegeria mobilis sp. nov., a member of the Alphaproteobacteria isolated in Japan and Palau. Int J Syst Evol Microbiol 57 1304–1309 [View Article] [PubMed].
     [Google Scholar]
  37. Oh K. H., Jung Y. T., Oh T. K., Yoon J. H. ( 2011;). Ruegeria faecimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 61 1182–1188 [View Article] [PubMed].
     [Google Scholar]
  38. Park S., Yoon J. H. ( 2012;). Ruegeria arenilitoris sp. nov., isolated from the seashore sand around a seaweed farm. Antonie van Leeuwenhoek 102 581–589 [View Article] [PubMed].
     [Google Scholar]
  39. Park S., Lee M. H., Yoon J. H. ( 2013;). Oceanicola litoreus sp. nov., an alphaproteobacterium isolated from the seashore sediment. Antonie van Leeuwenhoek 103 859–866 [View Article] [PubMed].
     [Google Scholar]
  40. Park S., Won S. M., Kim H., Park D. S., Yoon J. H. ( 2014a;). Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 64 2969–2974 [View Article] [PubMed].
     [Google Scholar]
  41. Park S., Park J. M., Lee K. C., Bae K. S., Yoon J. H. ( 2014b;). Boseongicola aestuarii gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 64 2618–2624 [View Article] [PubMed].
     [Google Scholar]
  42. Rathgeber C., Yurkova N., Stackebrandt E., Schumann P., Beatty J. T., Yurkov V. ( 2005;). Roseicyclus mahoneyensis gen. nov., sp. nov., an aerobic phototrophic bacterium isolated from a meromictic lake. Int J Syst Evol Microbiol 55 1597–1603 [View Article] [PubMed].
     [Google Scholar]
  43. Romanenko L. A., Tanaka N., Svetashev V. I., Kalinovskaya N. I. ( 2012;). Poseidonocella pacifica gen. nov., sp. nov. and Poseidonocella sedimentorum sp. nov., novel alphaproteobacteria from the shallow sandy sediments of the Sea of Japan. Arch Microbiol 194 113–121 [View Article] [PubMed].
     [Google Scholar]
  44. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 [PubMed].
     [Google Scholar]
  45. Srinivas T. N. R., Kumar P. A., Sasikala Ch., Ramana ChV. . ( 2007;). Rhodovulum imhoffii sp. nov. Int J Syst Evol Microbiol 57 228–232 [View Article] [PubMed].
     [Google Scholar]
  46. Suzuki T., Muroga Y., Takahama M., Nishimura Y. ( 2000;). Roseibium denhamense gen. nov., sp. nov. and Roseibium hemelinense sp. nov., aerobic bacteriochlorophyll-containing bacteria isolated from the east and west coasts of Australia. Int J Syst Evol Microbiol 50 2151–2156 [View Article] [PubMed].
     [Google Scholar]
  47. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed].
     [Google Scholar]
  48. Uchino Y., Hirata A., Yokota A., Sugiyama J. ( 1998;). Reclassification of marine Agrobacterium species: Proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44 201–210 [View Article] [PubMed].
     [Google Scholar]
  49. Uchino Y., Hamada T., Yokota A. ( 2002;). Proposal of Pseudorhodobacter ferrugineus gen. nov., comb. nov., for a non-photosynthetic marine bacterium, Agrobacterium ferrugineum, related to the genus Rhodobacter . J Gen Appl Microbiol 48 309–319 [View Article] [PubMed].
     [Google Scholar]
  50. Vandecandelaere I., Nercessian O., Segaert E., Achouak W., Faimali M., Vandamme P. ( 2008;). Ruegeria scottomollicae sp. nov., isolated from a marine electroactive biofilm. Int J Syst Evol Microbiol 58 2726–2733 [View Article] [PubMed].
     [Google Scholar]
  51. Wang Y. X., Wang Z. G., Liu J. H., Chen Y. G., Zhang X. X., Wen M. L., Xu L. H., Peng Q., Cui X. L. ( 2009;). Sediminimonas qiaohouensis gen. nov., sp. nov., a member of the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 59 1561–1567 [View Article] [PubMed].
     [Google Scholar]
  52. Wang D., Liu H., Zheng S., Wang G. ( 2014;). Paenirhodobacter enshiensis gen. nov., sp. nov., a non-photosynthetic bacterium isolated from soil, and emended descriptions of the genera Rhodobacter and Haematobacter . Int J Syst Evol Microbiol 64 551–558 [View Article] [PubMed].
     [Google Scholar]
  53. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 697–703 [PubMed].
     [Google Scholar]
  54. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16 176–178.
     [Google Scholar]
  55. Yi H., Lim Y. W., Chun J. ( 2007). Taxonomic evaluation of the genera Ruegeria and Silicibacter: a proposal to transfer the genus Silicibacter Petursdottir and Kristjansson 1999 to the genus Ruegeria Uchino et al. 1999. Int J Syst Evol Microbiol 57 815–819 [View Article] [PubMed].
     [Google Scholar]
  56. Yoon J.-H., Kang S.-J., Lee M.-H., Oh T.-K. ( 2007;). Description of Sulfitobacter donghicola sp. nov., isolated from seawater of the East Sea in Korea, transfer of Staleya guttiformis Labrenz et al. 2000 to the genus Sulfitobacter as Sulfitobacter guttiformis comb. nov. and emended description of the genus Sulfitobacter . Int J Syst Evol Microbiol 57 1788–1792 [View Article] [PubMed].
     [Google Scholar]
  57. Yoon J. H., Kang S. J., Lee J. S., Oh T. K. ( 2009a;). Lutimaribacter saemankumensis gen. nov., sp. nov., isolated from a tidal flat of the Yellow Sea. Int J Syst Evol Microbiol 59 48–52 [View Article] [PubMed].
     [Google Scholar]
  58. Yoon J. H., Kang S. J., Lee S. Y., Oh K. H., Oh T. K. ( 2009b;). Seohaeicola saemankumensis gen. nov., sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 59 2675–2679 [View Article] [PubMed].
     [Google Scholar]
  59. Yoon J. H., Kang S. J., Jung Y. T., Oh T. K. ( 2010;). Gaetbulicola byunsanensis gen. nov., sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 60 196–199 [View Article] [PubMed].
     [Google Scholar]
  60. Yoon J.-H., Kang S.-J., Lee S.-Y., Jung Y.-T., Lee J.-S., Oh T.-K. ( 2012;). Marivita hallyeonensis sp. nov., isolated from seawater, reclassification of Gaetbulicola byunsanensis as Marivita byunsanensis comb. nov. and emended description of the genus Marivita Hwang et al. 2009. Int J Syst Evol Microbiol 62 839–843 [View Article] [PubMed].
     [Google Scholar]
  61. Yoon J. H., Kang S. J., Lee J. S. ( 2013a;). Marivita geojedonensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63 423–427 [View Article] [PubMed].
     [Google Scholar]
  62. Yoon J. H., Lee S. Y., Jung Y. T., Lee J. S., Lee K. C. ( 2013b;). Litorisediminicola beolgyonensis gen. nov., sp. nov., isolated from a coastal sediment. Int J Syst Evol Microbiol 63 2025–2031 [View Article] [PubMed].
     [Google Scholar]
  63. Yuan J., Lai Q., Wang B., Sun F., Liu X., Du Y., Li G., Gu L., Zheng T., Shao Z. ( 2009;). Oceanicola pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium. Int J Syst Evol Microbiol 59 1158–1161 [View Article] [PubMed].
     [Google Scholar]
  64. Zheng Q., Chen C., Wang Y. N., Jiao N. ( 2010;). Oceanicola nitratireducens sp. nov., a marine alphaproteobacterium isolated from the South China Sea. Int J Syst Evol Microbiol 60 1655–1659 [View Article] [PubMed].
     [Google Scholar]
  65. Zhong Z.-P., Liu Y., Liu H.-C., Wang F., Zhou Y.-G., Liu Z.-P. ( 2014;). Roseibium aquae sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol 64 2812–2818 [View Article] [PubMed].
     [Google Scholar]
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Lacimonas salitolerans gen. nov., sp. nov., isolated from surface water of a saline lake
Int J Syst Evol Microbiol 65, 4550 (2015); https://doi.org/10.1099/ijsem.0.000611
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