@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000625, author = "Nurkanto, Arif and Lisdiyanti, Puspita and Hamada, Moriyuki and Ratnakomala, Shanti and Shibata, Chiyo and Tamura, Tomohiko", title = "Cryptosporangium cibodasense sp. nov., isolated from leaf litter in Indonesia", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2015", volume = "65", number = "Pt_12", pages = "4632-4637", doi = "https://doi.org/10.1099/ijsem.0.000625", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000625", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel actinomycete strain, designated LIPI11-2-Ac046T, was isolated from a leaf litter sample obtained from Cibodas Botanical Garden, West Java, Indonesia, using the rehydration and centrifugation method. The taxonomic status of this organism was established using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain LIPI11-2-Ac046T had the closest sequence similarities with members of the genus Cryptosporangium (97.99–98.90 %). The strain grew well on ISP 4 and ISP 5 media and formed sporangia. Spores of this strain were motile. The strain grew in the presence of 0–2 % (w/v) NaCl and the temperature range of 15–28 °C. The cell-wall hydrolysate contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysate contained mannose, glucose, galactose, ribose and xylose, together with one unidentified O-methyl-pentose. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8), and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were C18 : 1ω9c, iso-C16 : 0, C16 : 0 and C17 : 1ω9c. These phenotypic characteristics corresponded to those of the genus Cryptosporangium. Meanwhile, the results of DNA–DNA hybridization as well as physiological and biochemical analyses distinguished strain LIPI11-2-Ac046T from known members of the genus Cryptosporangium. On the basis of these data, it is proposed that strain LIPI11-2-Ac046T represents a novel species of the genus Cryptosporangium, with the name Cryptosporangium cibodasense sp. nov. The type strain is LIPI11-2-Ac046T ( = InaCC A457T = NBRC 110976T).", }