RT Journal Article SR Electronic(1) A1 Nurkanto, Arif A1 Lisdiyanti, Puspita A1 Hamada, Moriyuki A1 Ratnakomala, Shanti A1 Shibata, Chiyo A1 Tamura, TomohikoYR 2015 T1 Cryptosporangium cibodasense sp. nov., isolated from leaf litter in Indonesia JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_12 SP 4632 OP 4637 DO https://doi.org/10.1099/ijsem.0.000625 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete strain, designated LIPI11-2-Ac046T, was isolated from a leaf litter sample obtained from Cibodas Botanical Garden, West Java, Indonesia, using the rehydration and centrifugation method. The taxonomic status of this organism was established using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain LIPI11-2-Ac046T had the closest sequence similarities with members of the genus Cryptosporangium (97.99–98.90 %). The strain grew well on ISP 4 and ISP 5 media and formed sporangia. Spores of this strain were motile. The strain grew in the presence of 0–2 % (w/v) NaCl and the temperature range of 15–28 °C. The cell-wall hydrolysate contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysate contained mannose, glucose, galactose, ribose and xylose, together with one unidentified O-methyl-pentose. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8), and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were C18 : 1ω9c, iso-C16 : 0, C16 : 0 and C17 : 1ω9c. These phenotypic characteristics corresponded to those of the genus Cryptosporangium. Meanwhile, the results of DNA–DNA hybridization as well as physiological and biochemical analyses distinguished strain LIPI11-2-Ac046T from known members of the genus Cryptosporangium. On the basis of these data, it is proposed that strain LIPI11-2-Ac046T represents a novel species of the genus Cryptosporangium, with the name Cryptosporangium cibodasense sp. nov. The type strain is LIPI11-2-Ac046T ( = InaCC A457T = NBRC 110976T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000625