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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_12

gen. nov., sp. nov., an endophytic actinomycete of the family Free

Abstract

A novel actinomycete strain, designated KLBMP 1356, was isolated from the root of halophyte Lour. collected from the coastal area of Jiangsu province, PR China. The isolate was characterized using a polyphasic approach. Comparative analysis of the 16S rRNA gene sequence indicated that strain KLBMP 1356 was phylogenetically related to members of the family and formed a distinct monophyletic clade between the genera (93.1–94.7 % 16S rRNA gene sequence similarity), (93.6–95.1 %) and (93.2–94.3 %). The isolate displayed long spore chains containing rod-shaped and smooth-surfaced spores. Strain KLBMP 1356 contained -diaminopimelic acid as the diagnostic diamino acid, and galactose, arabinose and glucose as the whole-cell sugars. The major menaquinone was MK-9(H) and the fatty acid profile was characterized by the predominance of iso-C, Cω8, Cω6 and C. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, unknown aminophospholipids and an unknown glycolipid. Mycolic acids were not present. The G+C content of the genomic DNA was 67.2 mol%. On the basis of the evidence from this polyphasic study, strain KLBMP 1356 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is KLBMP 1356 ( = DSM 45765 = NBRC 109361).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China
  • the opening project of the State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
  • Qing Lan Project of Jiangsu Province (2014)
  • the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
© 2015 IUMS
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2015-12-01
2024-04-19
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Tamaricihabitans halophyticus gen. nov., sp. nov., an endophytic actinomycete of the family Pseudonocardiaceae
Int J Syst Evol Microbiol 65, 4662 (2015); https://doi.org/10.1099/ijsem.0.000628
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