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f sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil

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  • Authors: Jae-Chan Lee1​ ,  Young-Sook Kim2​ ,3​ ,  Bong-Sik Yun2​ , Kyung-Sook Whang1​ ,4​
    • VIEW AFFILIATIONS
    • 1​ 1​ Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-318, Republic of Korea 2​ 2​ Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, 79 Gobong-ro, Iksan-si 570-752, Republic of Korea 3​ 3​ Research Center for Biobased Chemistry, Eco-friendly New Materials Research Group, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, Republic of Korea 4​ 4​ Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-318, Republic of Korea
  • Bong-Sik Yun [email protected]
  • First Published Online: 01 December 2015, International Journal of Systematic and Evolutionary Microbiology 65: 4792-4799, doi: 10.1099/ijsem.0.000650
  • Subject: NEW TAXA - Proteobacteria
  • Cover date:
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  • A Gram-stain-negative, halotolerant and alkalitolerant bacterium, designated strain BH103, was isolated from saltern soil in Gomso, Korea. Cells of strain BH103 were strictly aerobic, motile, straight rods and grew at pH 7.0–10.8 (optimum, pH 8.5), at 10–55 °C (optimum, 28 °C) and at salinities of 0–23 % (w/v) NaCl (optimum, 14 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH103 belongs to the genus , showing highest sequence similarity to LC1 (97.7 %), Eplume1 (97.7 %), III (97.7 %), CRSS (97.7 %), TYRC17 (97.5 %), BH1 (97.2 %) and Esulfide1 (96.2 %). The predominant ubiquinone was Q-9. The major fatty acids were Cω7, Cω7 and/or iso-C 2-OH, C and C 3-OH. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and an unknown phospholipid. The DNA G+C content of this novel isolate was 54.7 mol%. DNA–DNA relatedness between strain BH103 and KACC 16615, KCTC 2888, KCTC 2889, KCTC 22844, DSM 19074, JCM 16411 and DSM 15722 was 45, 41, 39, 32, 38, 45 and 35 %, respectively. On the basis of polyphasic analysis from this study, strain BH103 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BH103 ( = KACC 17609 = NBRC 109914 = NCAIM B 02528).

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and 23S rRNA gene sequences of strain BH103 are KF963827 and LC053990, respectively.

  • One supplementary table and five supplementary figures are available with the online Supplementary Material.

© 2015 IUMS | Published by the Microbiology Society

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