1887
  1. Home
  2. Publications
  3. International Journal of Systematic and Evolutionary Microbiology
  4. Volume 65, Issue 12
  5. Article

f sp. nov., isolated from acid mine water

HTML
66.05 Kb
PDF
211.20 Kb
  • Authors: Bo Zhang1​ ,2​ ,  Yu-Fan Wu1​ ,2​ ,  Jin-Long Song1​ ,  Zhong-Sheng Huang3​ ,  Bao-Jun Wang1​ ,  Shuang-Jiang Liu1​ , Cheng-Ying Jiang1​
    • VIEW AFFILIATIONS
    • 1​ 1​ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China 2​ 2​ Graduate School of Chinese Academy of Science, Beijing 100049, PR China 3​ 3​ State Key Laboratory of Comprehensive Utilization of Low-Grade Refractory Gold Ores (Zijin Mining Group Co., Ltd), , Fujian 364200, PR China
  • Cheng-Ying Jiang [email protected]
  • First Published Online: 01 December 2015, International Journal of Systematic and Evolutionary Microbiology 65: 4915-4920, doi: 10.1099/ijsem.0.000695
  • Subject: ICSP Matters
  • Cover date:
Preview this:
Free Preview thumbnail Magnify
Zoom in
Zoomout

sp. nov., isolated from acid mine water, Page 1 of 1

| /docserver/preview/fulltext/ijsem/65/12/4915_ijsem000695-1.gif

  • Two novel, Gram-stain-variable, moderately thermophilic, acidophilic, rod-shaped, endospore-forming bacteria, G45-16 and G45-17, were isolated from acid mine water of Zijin copper mine in Fujian Province, China. Phylogenetic analysis of 16S rRNA gene sequences showed that they were closely related to ATCC 49025 with sequence similarities of 96.8 %. Cells grew aerobically at 20–45 °C (optimum, 40 °C), at pH 2.5–5.5 (optimum, pH 3.5) and in the presence of 0–4.0 % (w/v) NaCl. Strains contained MK-7 as the major menaquinone and the major cellular fatty acids were ω-cyclohexane C and ω-cyclohexane C. The DNA G+C content was 51.3 and 49.8 mol% ( ) for G45-16 and G45-17, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons with their relatives and DNA–DNA relatedness values, it is concluded that strains G45-16 and G45-17 represent a novel species within the genus , for which the name sp. nov. is proposed; the type strain is G45-16 ( = CGMCC 1.15049 = NBRC 111483).

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains G45-16 and G45-17 are KR090679 and KR090680, respectively.

  • One supplementary table and one supplementary figure are available with the online Supplementary Material.

© 2015 IUMS | Published by the Microbiology Society

  • Albuquerque L. , Rainey F. A. , Chung A. P. , Sunna A. , Nobre M. F. , Grote R. , Antranikian G. , da Costa M. S. . ( 2000;). Alicyclobacillus hesperidum sp. nov. and a related genomic species from solfataric soils of São Miguel in the Azores. Int J Syst Evol Microbiol 50: 451––457 [CrossRef] [PubMed].
     [Google Scholar]
  • Darland G. , Brock T. D. . ( 1971;). Bacillus acidocaldarius sp.nov., an acidophilic thermophilic spore-forming bacterium. J Gen Microbiol 67: 9––15 [CrossRef].
     [Google Scholar]
  • De Ley J. , Cattoir H. , Reynaerts A. . ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12: 133––142 [CrossRef] [PubMed].
     [Google Scholar]
  • Deinhard G. , Blanz P. , Poralla K. , Altan E. . ( 1987a;). Bacillus acidoterrestris sp. nov., a new thermotolerant acidophile isolated from different soils. Syst Appl Microbiol 10: 47––53 [CrossRef].
     [Google Scholar]
  • Deinhard G. , Saar J. , Krischke W. , Poralla K. . ( 1987b;). Bacillus cycloheptanicus sp. nov., a new thermoacidophile containing ω-cycloheptane fatty acids. Syst Appl Microbiol 10: 68––73 [CrossRef].
     [Google Scholar]
  • Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783––791 [CrossRef].
     [Google Scholar]
  • Felsenstein J. . phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA (1993). .
  • Fitch W. M. . ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406––416 [CrossRef].
     [Google Scholar]
  • Goto K. , Matsubara H. , Mochida K. , Matsumura T. , Hara Y. , Niwa M. , Yamasato K. . ( 2002;). Alicyclobacillus herbarius sp. nov., a novel bacterium containing ω-cycloheptane fatty acids, isolated from herbal tea. Int J Syst Evol Microbiol 52: 109––113 [CrossRef] [PubMed].
     [Google Scholar]
  • Goto K. , Mochida K. , Asahara M. , Suzuki M. , Kasai H. , Yokota A. . ( 2003;). Alicyclobacillus pomorum sp. nov., a novel thermo-acidophilic, endospore-forming bacterium that does not possess ω-alicyclic fatty acids, and emended description of the genus Alicyclobacillus . Int J Syst Evol Microbiol 53: 1537––1544 [CrossRef] [PubMed].
     [Google Scholar]
  • Goto K. , Mochida K. , Kato Y. , Asahara M. , Fujita R. , An S. Y. , Kasai H. , Yokota A. . ( 2007;). Proposal of six species of moderately thermophilic, acidophilic, endospore-forming bacteria: Alicyclobacillus contaminans sp. nov., Alicyclobacillus fastidiosus sp. nov., Alicyclobacillus kakegawensis sp. nov., Alicyclobacillus macrosporangiidus sp. nov., Alicyclobacillus sacchari sp. nov. and Alicyclobacillus shizuokensis sp. nov. Int J Syst Evol Microbiol 57: 1276––1285 [CrossRef] [PubMed].
     [Google Scholar]
  • Guo X. , You X. Y. , Liu L. J. , Zhang J. Y. , Liu S. J. , Jiang C. Y. . ( 2009;). Alicyclobacillus aeris sp. nov., a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine. Int J Syst Evol Microbiol 59: 2415––2420 [CrossRef] [PubMed].
     [Google Scholar]
  • Hippchen B. , Röll A. , Poralla K. . ( 1981;). Occurrence in soil of thermo-acidophilic bacilli possessing ω-cyclohexane fatty acids and hopanoids. Arch Microbiol 129: 53––55 [CrossRef].
     [Google Scholar]
  • Hiraishi A. , Inagaki K. , Tanimoto Y. , Iwasaki M. , Kishimoto N. , Tanaka H. . ( 1997;). Phylogenetic characterization of a new thermoacidophilic bacterium isolated from hot springs in Japan. J Gen Appl Microbiol 43: 295––304 [CrossRef] [PubMed].
     [Google Scholar]
  • Hudson J. A. , Morgan H. W. , Daniel R. M. . ( 1986;). A numerical classification of some Thermus isolates. J Gen Microbiol 132: 531––540.
     [Google Scholar]
  • Huss V. A. , Festl H. , Schleifer K. H. . ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4: 184––192 [CrossRef] [PubMed].
     [Google Scholar]
  • Imperio T. , Viti C. , Marri L. . ( 2008;). Alicyclobacillus pohliae sp. nov., a thermophilic, endospore-forming bacterium isolated from geothermal soil of the north-west slope of Mount Melbourne (Antarctica). Int J Syst Evol Microbiol 58: 221––225.[CrossRef]
    [Google Scholar]
  • Jiang C. Y. , Liu Y. , Liu Y. Y. , You X. Y. , Guo X. , Liu S. J. . ( 2008;). Alicyclobacillus ferrooxydans sp. nov., a ferrous-oxidizing bacterium from solfataric soil. Int J Syst Evol Microbiol 58: 2898––2903 [CrossRef] [PubMed].
     [Google Scholar]
  • Jiang C. , Liu Y. , Liu Y. , Guo X. , Liu S. J. . ( 2009;). Isolation and characterization of ferrous- and sulfur-oxidizing bacteria from Tengchong solfataric region, China. J Environ Sci (China) 21: 1247––1252 [CrossRef] [PubMed].
     [Google Scholar]
  • Karavaiko G. I. , Kondrat'eva T. F. , Tsaplina I. A. , Egorova M. A. , Krasil'nikova E. N. , Zakharchuk L. M. . ( 2005;). Reclassification of ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended description of the genus Alicyclobacillus . Int J Syst Evol Microbiol 55: 941––947.[CrossRef]
    [Google Scholar]
  • Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716––721 [CrossRef] [PubMed].
     [Google Scholar]
  • Kusube M. , Sugihara A. , Moriwaki Y. , Ueoka T. , Shimane Y. , Minegishi H. . ( 2014;). Alicyclobacillus cellulosilyticus sp. nov., a thermophilic, cellulolytic bacterium isolated from steamed Japanese cedar chips from a lumbermill. Int J Syst Evol Microbiol 64: 2257––2263.[CrossRef]
    [Google Scholar]
  • Marmur J. , Doty P. . ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109––118 [CrossRef] [PubMed].
     [Google Scholar]
  • Matsubara H. , Goto K. , Matsumura T. , Mochida K. , Iwaki M. , Niwa M. , Yamasato K. . ( 2002;). Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, ω-alicyclic fatty acid-containing bacterium isolated from acidic beverages. Int J Syst Evol Microbiol 52: 1681––1685 [PubMed].
     [Google Scholar]
  • Nakano C. , Takahashi N. , Tanaka N. , Okada S. . ( 2015;). Alicyclobacillus dauci sp. nov., a slightly thermophilic, acidophilic bacterium isolated from a spoiled mixed vegetable and fruit juice product. Int J Syst Evol Microbiol 65: 716––722 [CrossRef] [PubMed].
     [Google Scholar]
  • Nicolaus B. , Improta R. , Manca C. M. , Lama L. , Esposito E. , Gambacorta A. . ( 1998;). Alicyclobacilli from an unexplored geothermal soil in Antarctica: Mount Rittmann. Polar Biol 19: 133––141 [CrossRef].
     [Google Scholar]
  • Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406––425 [PubMed].
     [Google Scholar]
  • Schaeffer A. B. , Fulton M. D. . ( 1933;). A simplified method of staining endospores. Science 77: 194 [CrossRef] [PubMed].
     [Google Scholar]
  • Simbahan J. , Drijber R. , Blum P. . ( 2004;). Alicyclobacillus vulcanalis sp. nov., a thermophilic, acidophilic bacterium isolated from Coso Hot Springs, California, USA. Int J Syst Evol Microbiol 54: 1703––1707 [CrossRef] [PubMed].
     [Google Scholar]
  • Smibert R. M. , Krieg N. R. . ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607––654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  • Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725––2729 [CrossRef] [PubMed].
     [Google Scholar]
  • Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876––4882 [CrossRef] [PubMed].
     [Google Scholar]
  • Tsuruoka N. , Isono Y. , Shida O. , Hemmi H. , Nakayama T. , Nishino T. . ( 2003;). Alicyclobacillus sendaiensis sp. nov., a novel acidophilic, slightly thermophilic species isolated from soil in Sendai, Japan. Int J Syst Evol Microbiol 53: 1081––1084 [CrossRef] [PubMed].
     [Google Scholar]
  • Wayne L. , Brenner D. , Colwell R. , Grimont P. , Kandler O. , Krichevsky M. , Moore L. , Moore W. , Murray R. , other authors . ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463––464 [CrossRef].
     [Google Scholar]
  • Wisotzkey J. D. , Jurtshuk P. Jr , Fox G. E. , Deinhard G. , Poralla K. . ( 1992;). Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int J Syst Bacteriol 42: 263––269 [CrossRef] [PubMed].
     [Google Scholar]
http://sgm.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000695
Loading

Supplementary Data

 Data loading....

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000695
2015-12-01
2019-02-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4915.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000695&mimeType=html&fmt=ahah

Author and Article Information

 Figure data loading....

Footnotes:

Validated antibodies in this article

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error