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- Volume 66 Number 2
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f Massilia violacea sp. nov., isolated from riverbank soil
- Authors: Salvador Embarcadero-Jiménez1 , Álvaro Peix2,3 , José Mariano Igual2,3 , Flor N. Rivera-Orduña1 , En Tao Wang1
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1 1Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico 2 2Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain 3 3Unidad Asociada Grupo de Interacción Planta-Microorganismo, Universidad de Salamanca-IRNASA (CSIC), Salamanca, Spain
- Correspondence En Tao Wang [email protected]
- First Published Online: 01 February 2016, International Journal of Systematic and Evolutionary Microbiology 66: 707-711, doi: 10.1099/ijsem.0.000776
- Subject: NEW TAXA - Proteobacteria
- Cover date:




Massilia violacea sp. nov., isolated from riverbank soil, Page 1 of 1
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A bacterial strain designated CAVIOT was isolated during the course of a study of culturable bacteria in a riverbank soil sample from Tlaxcala, Mexico. The strain was subjected to a polyphasic taxonomic characterization. Strain CAVIOT was aerobic, Gram-stain-negative, non-spore-forming and rod-shaped. Colonies grown on R2A agar at 28 °C were pale violet, mucoid, rounded, smooth and glossy. The strain was motile and catalase- and oxidase-positive, and maximum growth temperature was 35 °C. Strain CAVIOT was classified within the genus Massilia as its 16S rRNA gene sequence was closely related to those of Massilia umbonata LP01T (97.5 % similarity), Massilia dura 16T (97.2 %) and Massilia plicata 76T (97.1 %). The predominant respiratory quinone was Q8. The major fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C16 : 0 and summed feature 8 (C18 : 1ω7c/C18 : 1ω6c). The predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and an unknown phospholipid. The DNA G+C content was 65.0 mol% (Tm). DNA–DNA hybridization results showed values below 25 % with respect to the type strains of the closest related species. Therefore, strain CAVIOT can be differentiated from previously described species of the genus Massilia and represents a novel species, for which the name Massilia violacea sp. nov. is proposed. The type strain is CAVIOT ( = CECT 8897T = LMG 28941T).
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of CAVIOT is KT003718.
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One supplementary table and three supplementary figures are available with the online Supplementary Material.
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Abbreviations: ML maximum-likelihood NJ neighbour-joining
© 2015 IUMS | Published by the Microbiology Society
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