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f sp. nov., isolated from the liverwort

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  • A Gram-stain-positive, aerobic, motile, rod-shaped, endospore-forming bacterium, designated strain R55, was isolated from the liverwort growing at Gawalong glacier, Tibet, and characterized by using a polyphasic taxonomic approach. The major fatty acids of strain R55 were anteiso-C, C and iso-C. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the predominant polar lipids and occurred along with two unidentified aminophospholipids, one unidentified phospholipid and one unidentified aminolipid. Strain R55 contained MK-7 as the dominant menaquinone and -diaminopimelic acid as the major diagnostic diamino acid in the cell-wall peptidoglycan. The G+C content of the genomic DNA was 36.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain R55 was affiliated to species of the genus , and was related most closely to CY1 (97.1 % similarity). However, the DNA–DNA relatedness between this strain and strain R55 was only 44.1 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, strain R55 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R55 ( = CGMCC 1.15043 = DSM 29850).

  • The GenBank accession numbers for the 16S rRNA and gene sequences of strain R55 are KP056549 and KP069474, respectively. The GenBank accession number for the gene sequence of CCTCC AB 2013369 ( = CY1) is KT267165.

  • Four supplementary figures are available with the online Supplementary Material.

  • Abbreviations:
    ML maximum-likelihood
    NJ neighbour-joining.

© 2015 IUMS | Published by the Microbiology Society

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2019-02-20
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