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f sp. nov. and sp. nov.

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  • Authors: Yang Liu1 ,  Qiliang Lai1 ,  Juan Du1 , Zongze Shao1
    • VIEW AFFILIATIONS
    • 1 State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Collaborative Innovation Center of Deep Sea Biology; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PRChina
  • Zongze Shao [email protected]
  • First Published Online: 01 March 2016, International Journal of Systematic and Evolutionary Microbiology 66: 1193-1199, doi: 10.1099/ijsem.0.000856
  • Subject: NEW TAXA - Firmicutes and Related Organisms
  • Cover date:
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  • Two strains, designated DW5-4 and NH7I_1, were isolated from aquaculture water of a shrimp farm of Zhangzhou city in China and from surface sediment of the South China Sea, respectively. Cells of the strains were Gram-stain-positive, oxidase- and catalase-positive, rod-shaped and motile. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains belonged to the clade. 16S rRNA gene sequence similarity between strains DW5-4 and NH7I_1 was 99.8 %, and between each of the two strains and four type strains, ATCC 7061, FO-36b, 41KF2b and HYC-10, were above 99.4 %. The digital DNA–DNA hybridization value between the two novel strains was 41.6 %, and between each of the two strains and the four above-mentioned type strains were below 53.0 %. In both strains, the principal fatty acids were iso-C and anteiso-C, and the isoprenoid quinone was identified as MK-7 (100 %). The DNA G+C contents of strains DW5-4 and NH7I_1 were 41.4 and 41.3 mol%, respectively. Based on phenotypic and genotypic characteristics, two novel species of the genus are proposed: sp. nov., with DW5-4 ( = MCCC 1A08372 = LMG 27144 = KCTC 33531) as the type strain, and sp. nov., with NH7I_1 ( = MCCC 1A05787 = LMG 27697 = KCTC 33532) as the type strain.

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of DW5-4 and NH7I_1 are JX680133 and JX680098, respectively, those for the gene sequences are JX680210 and JX680175, respectively, and those for the draft genome sequences are JOTP00000000 and LGYN00000000, respectively.

  • One supplementary table and two supplementary figures are available with the online Supplementary Material.

  • Abbreviations:
    dDDH digital DNA–DNA hybridization
    MCCC Marine Culture Collection of China
    ME minimum-evolution
    ML maximum-likelihood
    NJ neighbour-joining

© 2015 IUMS | Published by the Microbiology Society

  • Branquinho R. , Meirinhos-Soares L. , Carriço J. A. , Pintado M. , Peixe L. V. . ( 2014;). Phylogenetic and clonality analysis of Bacillus pumilus isolates uncovered a highly heterogeneous population of different closely related species and clones. FEMS Microbiol Ecol 90: 689––698 [CrossRef] [PubMed].
     [Google Scholar]
  • Branquinho R. , Klein G. , Kämpfer P. , Peixe L. V. . ( 2015;). The status of the species Bacillus aerophilus and Bacillus stratosphericus. Request for an Opinion. Int J Syst Evol Microbiol 65: 1101 [CrossRef] [PubMed].
     [Google Scholar]
  • Lai Q. , Liu Y. , Shao Z. . ( 2012;). Genome sequence of Bacillus sp. strain HYC-10, isolated from intestinal tract contents from a marine fish (Mugil cephalus). J Bacteriol 194: 6991 [CrossRef] [PubMed].
     [Google Scholar]
  • Lai Q. , Liu Y. , Shao Z. . ( 2014;). Bacillus xiamenensis sp. nov., isolated from intestinal tract contents of a flathead mullet (Mugil cephalus). Antonie van Leeuwenhoek 105: 99––107 [CrossRef] [PubMed].
     [Google Scholar]
  • Liu Y. , Lai Q. , Dong C. , Sun F. , Wang L. , Li G. , Shao Z. . ( 2013;). Phylogenetic diversity of the Bacillus pumilus group and the marine ecotype revealed by multilocus sequence analysis. PLoS One 8: e80097 [CrossRef] [PubMed].
     [Google Scholar]
  • Liu Y. , Kumar N. R. , Lai Q. , Du J. , Dobritsa A. P. , Samadpour M. , Shao Z. . ( 2015a;). Identification of strains Bacillus aerophilus MTCC 7304T as Bacillus altitudinis and Bacillus stratosphericus MTCC 7305T as a Proteus sp. and the status of the species Bacillus aerius Shivaji et al. 2006. Request for an Opinion. Int J Syst Evol Microbiol 65: 3228––3232.[CrossRef]
    [Google Scholar]
  • Liu Y. , Lai Q. , Du J. , Shao Z. . ( 2015b;). Reclassification of Bacillus invictae as a later heterotypic synonym of Bacillus altitudinis . Int J Syst Evol Microbiol 65: 2769––2773 [CrossRef] [PubMed].
     [Google Scholar]
  • Meier-Kolthoff J. P. , Auch A. F. , Klenk H. P. , Göker M. . ( 2013;). Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14: 60 [CrossRef] [PubMed].
     [Google Scholar]
  • Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. P. , Parlett J. H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233––241 [CrossRef].
     [Google Scholar]
  • Satomi M. , La Duc M. T. , Venkateswaran K. . ( 2006;). Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces. Int J Syst Evol Microbiol 56: 1735––1740 [CrossRef] [PubMed].
     [Google Scholar]
  • Shivaji S. , Chaturvedi P. , Suresh K. , Reddy G. S. , Dutt C. B. , Wainwright M. , Narlikar J. V. , Bhargava P. M. . ( 2006;). Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes. Int J Syst Evol Microbiol 56: 1465––1473 [CrossRef] [PubMed].
     [Google Scholar]
  • Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731––2739 [CrossRef] [PubMed].
     [Google Scholar]
  • Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. , other authors . ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463––464 [CrossRef].
     [Google Scholar]
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