@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000861, author = "Tang, Biao and Yu, Yucong and Zhi, Xiaoyang and Yang, Lingling and Cen, Xufeng and Zhao, Guoping and Ding, Xiaoming", title = "Streptomyces yangpuensis sp. nov., isolated from soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "3", pages = "1224-1229", doi = "https://doi.org/10.1099/ijsem.0.000861", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000861", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel Gram-stain-positive strain with sandy aerial mycelium and golden yellow substrate mycelium, designated fd2-tbT, was isolated from a soil sample collected in Shanghai, China, and its taxonomic status was established by phylogenetic analysis. 16S rRNA gene sequence analysis showed that strain fd2-tbT belonged to the genus Streptomyces and was related to Streptomyces amritsarensis JCM 19660T (99.9 % 16S rRNA gene sequence similarity), Streptomyces flavotricini NBRC 12770T (99.9 %), Streptomyces polychromogenes NBRC 13072T (99.8 %), Streptomyces racemochromogenes NRRL B-5430T (99.7 %), Streptomyces globosus LMG 19896T (99.5 %), Streptomyces toxytricini NBRC 12823T (99.5 %) and Streptomyces katrae NBRC 13447T (99.3 %). The cell wall of strain fd2-tbT contained ll-diaminopimelic acid, and whole-cell sugars were identified as glucose and ribose. The menaquinones MK-9(H4), MK-9(H6) and MK-9(H8) were also detected. In addition, the polar lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, as well as five unidentified phospholipids, were detected. Major cellular fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and C16 : 0. DNA–DNA hybridization experiments showed that strain fd2-tbT exhibited 36.5 ± 0.6 %, 43.5 ± 2.0 %, 11.1 ± 1.3 %, 10.3 ± 3.1 %, 9.8 ± 1.9 %, 48.9 ± 3.9 % and 16.3 ± 1.7 % relatedness to S. amritsarensis JCM 40119660T, S. flavotricini NBRC 12770T, S. polychromogenes NBRC 13072T, S. racemochromogenes NRRL B-5430T, S. globosus LMG 19896T, S. toxytricini NBRC 12823T and S. katrae NBRC 13447T, respectively. Based on these analyses as well as some phenotypic differences, strain fd2-tbT is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces yangpuensis sp. nov. is proposed. The type strain is fd2-tbT ( = DSM 100336T = CGMCC 4.7256T).", }