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Volume 66, Issue 3

gen. nov., sp. nov., a new member of the family isolated from a biofilter in a recirculating aquaculture system Free

Abstract

A Gram-staining-negative bacterial strain (termed CZ-AZ5) was isolated from a biological filter in a marine recirculating aquaculture system in Tianjin, China. Its taxonomic status was determined using a polyphasic approach. CZ-AZ5 cells were non-spore-forming, non-motile rods, 0.6–0.7 μm wide and 3.0–3.7 μm long. CZ-AZ5 was strictly heterotrophic, aerobic, oxidase-negative and catalase-positive. Growth occurred in the temperature range 20–40 °C (optimal: 30 °C), pH range 6.0–8.5 (optimal: pH 7.5) and salinity range 0–5 % (w/v) NaCl (optimal: 1 %). In phylogenetic analyses based on 16S rRNA gene sequences, CZ-AZ5 was assigned to the family (phylum ) and was clustered with the genera and within this family. It showed highest sequence similarity to ‘ Haliscomenobacter calcifugiens’ (86.2 %), followed by ATCC 23119 (85.7 %) and T-3 (85.6 %). DNA G+C content was 40.1 mol%, the major menaquinone was MK-7, and the major cellular fatty acids (>10 %) were Cω7 and iso-C. Our phenotypic, chemotaxonomic and phylogenetic observations, taken together, led us to conclude that strain CZ-AZ5 represents a novel species and genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CZ-AZ5 ( = CGMCC 1.13179 = JCM 18886).

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2016-03-01
2024-04-24
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Membranicola marinus gen. nov., sp. nov., a new member of the family Saprospiraceae isolated from a biofilter in a recirculating aquaculture system
Int J Syst Evol Microbiol 66, 1275 (2016); https://doi.org/10.1099/ijsem.0.000870
/content/journal/ijsem/10.1099/ijsem.0.000870
/content/journal/ijsem/10.1099/ijsem.0.000870
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